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A Recessive Mutant of argonaute1b/gsnl4 Leads to Narrow Leaf, Small Grain Size and Low Seed Setting in Rice

SONG Mengqiu  RUAN Shuang  PENG Youlin  WANG Zhongwei  Jahan NOUSHIN  ZHANG Yu  CUI Yongtao  HU Haitao  JIANG Hongzhen  DING Shilin  SHEN Lan  GAO Zhenyu  HU Xingming  QIAN Qian  GUO Longbiao  
【摘要】:正A gsnl4 mutant characterized by small grain size, narrow leaf and low seed-setting rate was obtained by ethyl methane sulfonate(EMS) mutagenesis of a japonica rice variety Wuyunjing 21. Genetic analysis showed that gsnl4 is a loss-of-function mutant. A single-base mutation in gsnl4 resulted in the substitution of Ser to Asn in the Piwi domain of Os AGO1 b protein. CRISPR/Cas9-mediated editing of Os AGO1 b yielded a mutant phenotypically resembling gsnl4. Furthermore,

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