收藏本站
收藏 | 投稿 | 手机打开
二维码
手机客户端打开本文

DPHL:A DIA Pan-human Protein Mass Spectrometry Library for Robust Biomarker Discovery

Tiansheng Zhu  Yi Zhu  Yue Xuan  Huanhuan Gao  Xue Cai  Sander R.Piersma  Thang V.Pham  Tim Schelfhorst  Richard R.G.D.Haas  Irene V.Bijnsdorp  Rui Sun  Liang Yue  Guan Ruan  Qiushi Zhang  Mo Hu  Yue Zhou  Winan J.Van Houdt  Tessa Y.S.Le Large  Jacqueline Cloos  Anna Wojtuszkiewicz  Danijela Koppers-Lalic  Franziska Bottger  Chantal Scheepbouwer  Ruud H.Brakenhoff  Geert J.L.H.van Leenders  Jan N.M.Ijzermans  John W.M.Martens  Renske D.M.Steenbergen  Nicole C.Grieken  Sathiyamoorthy Selvarajan  Sangeeta Mantoo  Sze S.Lee  Serene J.Y.Yeow  Syed M.F.Alkaff  Nan Xiang  Yaoting Sun  Xiao Yi  Shaozheng Dai  Wei Liu  Tian Lu  Zhicheng Wu  Xiao Liang  Man Wang  Yingkuan Shao  Xi Zheng  Kailun Xu  Qin Yang  Yifan Meng  Cong Lu  Jiang Zhu  Jin'e Zheng  Bo Wang  Sai Lou  Yibei Dai  Chao Xu  Chenhuan Yu  Huazhong Ying  Tony K.Lim  Jianmin Wu  Xiaofei Gao  Zhongzhi Luan  Xiaodong Teng  Peng Wu  Shi'ang Huang  Zhihua Tao  Narayanan G.Iyer  Shuigeng Zhou  Wenguang Shao  Henry Lam  Ding Ma  Jiafu Ji  Oi L.Kon  Shu Zheng  Ruedi Aebersold  Connie R.Jimenez  Tiannan Guo  
【摘要】:To address the increasing need for detecting and validating protein biomarkers in clinical specimens,mass spectrometry(MS)-based targeted proteomic techniques,including the selected reaction monitoring(SRM),parallel reaction monitoring(PRM),and massively parallel dataindependent acquisition(DIA),have been developed.For optimal performance,they require the fragment ion spectra of targeted peptides as prior knowledge.In this report,we describe a MS pipeline and spectral resource to support targeted proteomics studies for human tissue samples.To build the spectral resource,we integrated common open-source MS computational tools to assemble a freely accessible computational workflow based on Docker.We then applied the workflow to generate DPHL,a comprehensive DIA pan-human library,from 1096 data-dependent acquisition(DDA) MS raw files for 16 types of cancer samples.This extensive spectral resource was then applied to a proteomic study of 17 prostate cancer(PCa) patients.Thereafter,PRM validation was applied to a larger study of 57 PCa patients and the differential expression of three proteins in prostate tumor was validated.As a second application,the DPHL spectral resource was applied to a study consisting of plasma samples from 19 diffuse large B cell lymphoma(DLBCL) patients and 18 healthy control subjects.Differentially expressed proteins between DLBCL patients and healthy control subjects were detected by DIA-MS and confirmed by PRM.These data demonstrate that the DPHL supports DIA and PRM MS pipelines for robust protein biomarker discovery.DPHL is freely accessible at https://www.iprox.org/page/project.html?id=IPX0001400000.

知网文化
【相似文献】
中国期刊全文数据库 前4条
1 ;Application of magnetic resonance imaging for monitoring stem cell transplantation for the treatment of cerebral ischemia[J];Neural Regeneration Research;2012年16期
2 Rushna Firdaus;Kallol Saha;Aritra Biswas;Provash Chandra Sadhukhan;;Current molecular methods for the detection of hepatitis C virus in high risk group population:A systematic review[J];World Journal of Virology;2015年01期
3 ;Study on non-invasively detecting of prenatal fetal ABO and Rh(D)blood groups by flow cytometry (FCM)[J];中国输血杂志;2001年S1期
4 ;Assessment of a method for detecting serum HBV DNA with HBV DNA probe labelled directly by alkaline phosphatase[J];Hepatobiliary & Pancreatic Diseases International;2003年04期
中国重要会议论文全文数据库 前1条
1 Nilini Ranbaduge;Henry Shion;Ying Qing Yu;Weibin Chen;;AN Integrated LC-MS Workflow for Subunit Core-fucosylation Characterization and Monitoring[A];第六届生物技术药物理化特性分析与质量研究技术研讨会资料汇编[C];2018年
 快捷付款方式  订购知网充值卡  订购热线  帮助中心
  • 400-819-9993
  • 010-62982499
  • 010-62783978