收藏本站
收藏 | 投稿 | 手机打开
二维码
手机客户端打开本文

Expression of an Episomal Vector in Developing Chicken Embryo and Chicks

Mu Yan-shuang  Wang Jia-qiang  Yin Zhi  Jiang Dan-dan  Li Hui  Liu Zhong-hua  
【摘要】:The domesticated chicken has important roles in basic and applied research. The vector based on scaffold matrix attachment region(S/MAR) appears to be sufficient to maintain long-term expression in an episomal state in various mammalian cells. To explore the practical use of episomal vector in transgene technology of agricultural chickens, we fused the S/MAR of the human β-interferon gene into the pEGFP vector and transduced it into chickens by sperm-mediated gene transfer(SMGT). PCR detection indicated the positive rate of transgene chickens was 60%. The RT-PCR detection and fluorescence observation confirmed the expression of the GFP and indicated the existence of the GFP during the chicken embryogenesis and fetal development. The PCR detection and rescue experiments confirmed the episomal state of the pEPI-EGFP in chick embryos and chicks. These results showed that the S/MAR-based vector could function properly in chicken embryos and was a practicable tool combined with the SMGT to study the development of chickens.

知网文化
【相似文献】
中国期刊全文数据库 前20条
1 ;Single nucleotide polymorphisms in chicken lmbr1 gene were associated with chicken growth and carcass traits[J];Science in China(Series C:Life Sciences);2007年01期
2 Ying Yang;Feng Sun;Chen Zhang;Hao Wang;Guoyao Wu;Zhenlong Wu;;Hypoxia promotes cell proliferation by modulating E2F1 in chicken pulmonary arterial smooth muscle cells[J];Journal of Animal Science and Biotechnology;2013年03期
3 ;Immunological changes of chicken infected with marek's disease virus[J];The Journal of Northeast Agricultural University(English Edition);1994年01期
4 李宁,陈永福;Ontogenetic expression of chicken growth hormone receptor gene[J];Progress in Natural Science;1995年06期
5 ;Folding patterns of chromatin in chicken erythrocyte studied by using AFM and TEM[J];Chinese Science Bulletin;1998年04期
6 S.Aritomi,N.Fujihara;Production of chicken chimeras by fusing blastodermal cells with electroporation[J];Asian Journal of Andrology;2000年04期
7 Lee Lucy F;Analyzing the H19- and T65-epitopes in 38 kd phosphory-lated protein of Marek's disease viruses and comparing chicken immunological reactions to viruses point-mutated in the epitopes[J];Science in China(Series C:Life Sciences);2004年01期
8 Rong-fa GUAN;Fei LYU;Xiao-qiang CHEN;Jie-qing MA;Han JIANG;Chao-geng XIAO;;Meat quality traits of four Chinese indigenous chicken breeds and one commercial broiler stock[J];Journal of Zhejiang University-Science B(Biomedicine & Biotechnology);2013年10期
9 童俊超,朱榴琴,杨福愉;V92A mutation altered folding propensity of chicken heart apocytochrome c[J];Chinese Science Bulletin;1996年05期
10 包素锦,万柱礼,常文瑞,桂璐璐,梁栋材,凌嵩,李林,许根俊,Simon J. Pilkis;Preliminary crystallographic analysis of recombinant chicken liver fructose-2,6-bisphosphatase[J];Science in China,Ser.B;1996年01期
11 范映辛,朱笠,周筠梅,邹承鲁;Sequential conformation change and activation of chicken liver dihydrofolate reductase in low concentration of guanidine hydrochloride[J];Science in China(Series C:Life Sciences);1997年02期
12 Fuminori Sato,Tomoki Soh,Masa-aki Hattori,Noboru Fujihara;Evaluation of deoxyribonuclease activity in seminal plasmaof ejaculated chicken semen[J];Asian Journal of Andrology;2003年03期
13 ;Insulin-like growth factor 2 as a candidate gene influencing growth and carcass traits and its bialleleic expression in chicken[J];Science in China(Series C:Life Sciences);2005年02期
14 ;The LTR of endogenous retrovirus ev21 retains promoter activity and exhibits tissue specific transcription in chicken[J];Chinese Science Bulletin;2009年24期
15 ;Adaptability of Fowlpox Virus in Chicken Embryo Passage Cells[J];Animal Husbandry and Feed Science;2011年04期
16 LUO Si-si;XIE Zhi-xun;XIE Zhi-qin;LIU Jia-bo;PANG Yao-shan;DENG Xian-wen;XIE Li-ji;FAN Qing;;Establishment of Indirect ELISA Method for Detection of Penton Protein of Fowl Adenovirus GroupⅠ[J];Animal Husbandry and Feed Science;2012年06期
17 ZHANG Xue-yu;SU Yi-jun;LI Guo-hui;TU Yun-jie;JIANG Yong-jun;YANG Fu-sheng;;Cluster Analysis of Laying Performance and Production Area Ecology of 11 kinds of Chicken[J];Animal Husbandry and Feed Science;2013年01期
18 Mu Yan-shuang;Wang Jia-qiang;Yin Zhi;Jiang Dan-dan;Li Hui;Liu Zhong-hua;;Expression of an Episomal Vector in Developing Chicken Embryo and Chicks[J];Journal of Northeast Agricultural University(English Edition);2013年03期
19 李同元,丁建芳,李林,许根俊;Preparation of monoclonal antibodies against chicken liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase and their effects on enzyme activities[J];Science in China(Series C:Life Sciences);1996年04期
20 GAO Gui-sheng;SHI Qiu-mei;ZHANG Yan-ying;SHEN Ping;TANG Sheng-ling;;The Influence of Chinese Medicinal Herb on Chicken Immune Organs and Small Intestinal Mucosa Immune Organization[J];Animal Husbandry and Feed Science;2012年05期
中国重要会议论文全文数据库 前10条
1 ;A novel SNP of visfatin gene and its associations with growth traits in chicken[A];中国动物遗传育种研究进展——第十五次全国动物遗传育种学术讨论会论文集[C];2009年
2 ;The effect of dehydroepiandrosterone on lipogenic gene mRNA expression in cultured primary chicken hepatocytes[A];全国动物生理生化第十一次学术交流会论文摘要汇编[C];2010年
3 ;Studies on the genetic differentiation of fifteen chicken breeds by mtDNA COI gene[A];第十二次全国畜禽遗传标记研讨会论文集[C];2010年
4 ;Use of DNA barcodes to identify indigenous chicken breeds in China[A];中国家禽业——机遇与挑战——第十三次全国家禽学术讨论会论文集[C];2007年
5 ;The effect of dehydroepiandrosterone on cell survival and hepatic lipid lipogenic gene mRNA expression in cultured primary chicken hepatocytes[A];中国生物化学与分子生物学会农业生物化学与分子生物学分会第八次学术研讨会论文集[C];2008年
6 ;Regulation of expression and activity of selenoenzymes by different forms and concentrations of selenium in primary cultured chicken hepatocytes[A];中国畜牧兽医学会家畜内科学分会第七届代表大会暨学术研讨会论文集(上册)[C];2011年
7 ;Contribution of the GABAergic pathway(s) to the correlated activities of chicken retinal ganglion cells[A];Proceedings of the 7th Biennial Meeting and the 5th Congress of the Chinese Society for Neuroscience[C];2007年
8 Pengcheng Jin;Xianwen Wu;Shouzhi Wang;Hui Zhang;Hui Li;;Bioinformatics and tissue expression profile analysis of DNAJB6 gene of chicken[A];第五届全国生物信息学与系统生物学学术大会论文集[C];2012年
9 Bohan Cheng;Li Leng;Hui Li;;Structural and functional analysis of chicken BMP4[A];第五届全国生物信息学与系统生物学学术大会论文集[C];2012年
10 SHU Jingting;XU Wenjuan;SHAN Yanju;SONG Chi;ZHU Wanqi;ZHANG Xueyu;LI Huifang;;Association Analysis of PGC-1αGene with Skeletal Muscle Fibre Types in Chicken[A];中国畜牧兽医学会家禽学分会第九次代表会议暨第十六次全国家禽学术讨论会论文集[C];2013年
中国博士学位论文全文数据库 前1条
1 WAEL M.K.ELFEIL (维尔);鸭、鹅PRRs的克隆、分析及分布研究[D];吉林大学;2012年
 快捷付款方式  订购知网充值卡  订购热线  帮助中心
  • 400-819-9993
  • 010-62982499
  • 010-62783978