收藏本站
收藏 | 投稿 | 手机打开
二维码
手机客户端打开本文

PH-responsive strontium nanoparticles for targeted gene therapy against mammary carcinoma cells

Athirah Bakhtiar  Ezharul Hoque Chowdhury  
【摘要】:Genetic intervention via the delivery of functional genes such as plasmid DNA(pDNA) and short-interfering RNA(siRNA) offers a great way to treat many single or multiple genetic defects effectively, including mammary carcinoma. Delivery of naked therapeutic genes or siRNAs is, however, short-lived due to biological clearance by scavenging nucleases and circulating monocytes. Low cellular internalization of negatively-charged nucleic acids further causes low transfection or silencing activity. Development of safe and effectual gene vectors is therefore undeniably crucial to the success of nucleic acid delivery. Inorganic nanoparticles have attracted considerable attention in the recent years due to their high loading capacity and encapsulation activity. Here we introduce strontium salt-based nanoparticles, namely, strontium sulfate, strontium sulfite and strontium fluoride as new inorganic nanocarriers. Generated strontium salt particles were found to be nanosized with high affinity towards negatively-charged pDNA and siRNA. Degradation of the particles was seen with a drop in pH, suggesting their capacity to respond to pH change and undergo dissolution at endosomal pH to release the genetic materials. While the particles are relatively nontoxic towards the cells, siRNA-loaded SrF_2 and SrSO_3 particles exerted superior transgene expression and knockdown activity of MAPK and AKT, leading to inhibition of their phosphorylation to a distinctive extent in both MCF-7 and 4T1 cells. Strontium salt nanoparticles have thus emerged as a promising tool for applications in cancer gene therapy.

知网文化
【相似文献】
中国期刊全文数据库 前18条
1 Hui Huang;Lingjun Kong;Ming Liu;Jie He;Wei Shuang;Yunhua Xu;Xian-He Bu;;Constructing bifunctional Co/MoC@N-C catalyst via an in-situ encapsulation strategy for efficient oxygen electrocatalysis[J];Journal of Energy Chemistry;2021年08期
2 刘霞;LI Juncan;HUANG Long;YANG Jin;WANG Yaowen;YANG Mengjia;汤明秀;邱彤;;Preparation and Evaluation of MPEG-PCL Polymeric Nanoparticles Against Gastric Cancer[J];Journal of Wuhan University of Technology(Materials Science);2020年06期
3 Zhihua Ma;Yuchun Wan;Wenyue Dong;Zhenjun Si;Qian Duan;Shiyang Shao;;Alkoxy encapsulation of carbazole-based thermally activated delayed fluorescent dendrimers for highly efficient solution-processed organic light-emitting diodes[J];Chinese Chemical Letters;2021年02期
4 Yujia Sun;Liwei Zheng;Yu Yang;Xu Qian;Ting Fu;Xiaowei Li;Zunyi Yang;He Yan;Cheng Cui;Weihong Tan;;Metal-Organic Framework Nanocarriers for Drug Delivery in Biomedical Applications[J];Nano-Micro Letters;2020年08期
5 Yingzhou WANG;Hao QI;Dongze ZHANG;Zixuan ZHAO;Chunyan ZHANG;;Preparation and in vitro Release of Donepezil Hydrochloride Microspheres[J];Medicinal Plant;2018年06期
6 Linlin Kong;Xinyi Jin;Dapeng Hu;Leyun Feng;Dong Chen;Hanying Li;;Functional delivery vehicle of organic nanoparticles in inorganic crystals[J];Chinese Chemical Letters;2019年12期
7 Yang Zhou;Aiping Fang;Fazhan Wang;Huili Li;Quansheng Jin;Lingjing Huang;Chunmei Fu;Jun Zeng;Zhaohui Jin;Xiangrong Song;;Core-shell lipid-polymer nanoparticles as a promising ocular drug delivery system to treat glaucoma[J];Chinese Chemical Letters;2020年02期
8 Cláudia Pina Costa;Jo?o Nuno Moreira;José Manuel Sousa Lobo;Ana Catarina Silva;;Intranasal delivery of nanostructured lipid carriers, solid lipid nanoparticles and nanoemulsions: A current overview of in vivo studies[J];Acta Pharmaceutica Sinica B;2021年04期
9 蔡秀兰;FU Datian;QU Ailan;;Effects of Processing Conditions on the Properties of Epoxy Resin Microcapsule[J];Journal of Wuhan University of Technology(Materials Science Edition);2015年04期
10 Anthony A Attama,Collins N Igbonekwu;;In vitro properties of surface-modified solid lipid microspheres containing an antimalarial drug:halofantrine[J];Asian Pacific Journal of Tropical Medicine;2011年04期
11 郑俊萍;;Preparation and Properties of Gelatin-Chitosan/Montmorillonite Drug-loaded Microspheres[J];Journal of Wuhan University of Technology(Materials Science Edition);2011年04期
12 Xu-Dong Shi;Pei-Jian Sun;Zhi-Hua Gan;;Preparation of Porous Polylactide Microspheres and Their Application in Tissue Engineering[J];Chinese Journal of Polymer Science;2018年06期
13 XU Haixing;ZHANG Yu;NIU Xiaoqian;WANG Ling;CHEN Hui;ZHANG Xi;TANG Qiuhan;HUANG Zhijun;LIU Hui;XU Peihu;;Preparation and in vitro Release Properties of Mercaptopurine Drug-loaded Magnetic Microspheres[J];Journal of Wuhan University of Technology(Materials Science Edition);2013年06期
14 仝维鋆;;MULTILAYERS AND POLY(ALLYLAMINE HYDROCHLORIDE)-GRAFTPOLY(ETHYLENE GLYCOL) MODIFIED BOVINE SERUM ALBUMIN NANOPARTICLES:IMPROVED STABILITY AND pH-RESPONSIVE DRUG DELIVERY[J];Chinese Journal of Polymer Science;2012年05期
15 Dayi Pan;Xiuli Zheng;Miao Chen;Qianfeng Zhang;Zhiqian Li;Zhenyu Duan;Qiyong Gong;Zhongwei Gu;Hu Zhang;Kui Luo;;Dendron-polymer hybrid mediated anticancer drug delivery for suppression of mammary cancer[J];Journal of Materials Science & Technology;2021年04期
16 Bing Hu;Qing-rong Huang;;BIOPOLYMER BASED NANO-DELIVERY SYSTEMS FOR ENHANCING BIOAVAILABILITY OF NUTRACEUTICALS[J];Chinese Journal of Polymer Science;2013年09期
17 ;Starch Modification for Slow Release of Herbicide Encapsulated with Concentrated Starch Paste[J];Wuhan University Journal of Natural Sciences;2007年03期
18 Wen-Ming Zhang;Jian Zhang;Zhu Qiao;Jun Yin;;Functionally Oriented Tumor Microenvironment Responsive Polymeric Nanoassembly: Engineering and Applications[J];Chinese Journal of Polymer Science;2018年03期
中国重要会议论文全文数据库 前7条
1 Yi Ke;Wen-Wen Ren;Ai-Min Bai;Yan-Jun Hu;;Encapsulation mechanism of metal-organic frameworks and bovine serum albumin[A];中国化学会第七届全国热分析动力学与热动力学学术会议会议摘要集[C];2019年
2 Jiankui Zhou;Li Zhang;Shaokui Cao;;Nonvolatile Resistive Switching Effect in Conjugated Polyrotaxanes[A];河南省化学会2020年学术年会论文摘要集[C];2020年
3 Ruibing Wang;;The use of cucurbiturils in pharmaceutical sciences[A];全国第十八届大环化学暨第十届超分子化学学术讨论会会议论文集(上)[C];2016年
4 吴桐;Guanhua WANG;Xiangrong ZHANG;;Preparation and Evaluation of Nanostructured Lipid Carriers loaded with Geniposide-Phospholipid Complex[A];2018年第十二届中国药物制剂大会论文集[C];2018年
5 汤昆山;范丽岩;张璇;刘春元;;Synthesis and Application of Dimolybdenum Complex with Double-Cavity Structure[A];第十六届全国金属有机化学学术讨论会论文集[C];2010年
6 Guanquan Lin;Xinhua Zhou;Jiaqi Lian;Huayao Chen;Hua Xu;Hongjun Zhou;;Preparation of pH-responsive avermectin/feather Keratin-hyaluronic Acid with Anti-UV and Sustained-release Properties[A];中国化工学会农药专业委员会第十八届年会论文集[C];2018年
7 王丹丹;张学农;;Folate-receptor Mediated Biomimetic Stock-like Nanoparticles for Dually Activated Anticancer Drug Delivery[A];2018年第十二届中国药物制剂大会论文集[C];2018年
中国博士学位论文全文数据库 前1条
1 Muhammad Akram;[D];浙江大学;2015年
 快捷付款方式  订购知网充值卡  订购热线  帮助中心
  • 400-819-9993
  • 010-62982499
  • 010-62783978