Genetic Diversity of MHC Gene and Molecular Phylogeny in Wanbei Cattle
【摘要】：[Objective] To investigate the genetic diversity, molecular phylogeny and origination in Wanbei cattle. [Method] Blood samples were collected from 50 Wanbei cattle and 14 local cattle breeds. According to the bovine MHC gene sequence, one pair of primers was designed and used for PCR amplification. The sequences of amplified MHC genes were aligned to detect MHC haplotypes and nucleotide polymorphisms. A phylogenetic tree based on the MHC gene was also constructed. [Result] A total of 24 polymorphic loci covering 7.86% of the entire length of the sequences and seven MHC haplotypes were detected. Among these polymorphic loci, there were 18 transitions, 1 tranversions and 5 transition / tranversion. The difference in nucleotide sequence was 0.26%-4.53% between the haplotypes. The nucleotide diversity (π value) and haplotype diversity (H) were 0.012-0.019 and 0.602-0.617, respectively. [Conclusion] Wanbei cattle do not have rich MHC genetic diversity. They have closer genetic relationship with Luxi cattle, and both are within the same sister group.