| | | | | 应用流式微球技术检测人泪液表皮生长因子 | | | 张艳莉;马璇;黄强;李兵;吴开力 | | | 目的将xMAP(flexiblemulti-analyteprofiling)技术和ELISA(enzyme-linkedimmunosorbentassay)应用到人泪液中的表皮生长因子(epidermalgrowthfactor,EGF)检测中,探讨xMAP技术在泪液检测中的应用前景。方法选取24例正常人(年龄18～33岁),在裂隙灯下取无刺激性泪液,用xMAP法检测泪液中EGF的浓度,与ELISA测定的结果比较,进行统计学分析。结果xMAP法检测泪液中EGF浓度为(4.32±2.57)ng/ml(范围0.96～9.76ng/ml),同一批样本经ELISA检测为(8.78±5.35)ng/ml(范围2.45～20.39ng/ml),两种方法的结果相关系数R2=0.8508(p=0.000)。结论xMAP法与ELISA法相关性良好,可以用于泪液中多种微量蛋白的检测。
【作者单位】：中山大学中山眼科中心;国家重点实验室;云南省第二人民医院眼科;中山大学中山眼科中心;国家重点实验室;中山大学中山眼科中心;国家重点实验室;中山大学中山眼科中心;国家重点实验室
【关键词】：表皮生长因子;泪液;酶联免疫吸附测定;流式微球技术
【基金】：国家自然科学基金团队项目(30321004);广东省社会发展计划重大专项(2004A30801001)
【分类号】：R77
【DOI】：cnki:ISSN:1006-4443.0.2006-11-017
【正文快照】：
人泪液量非常有限(15微升以下,正常分泌量<2微升/分钟)。许多泪液蛋白为低丰度蛋白质,常规检测方法(如ELISA)仅适于测定部分泪液蛋白,且每次只能分析一种蛋白。若开展临床泪液蛋白分析,必须具备高灵敏和高通量的泪液蛋白分析技术。迄今还没有将泪液蛋白分析应用到临床病人观测 | | | | | |  推荐 下载CAJ全文  下载PDF全文 | | | CAJViewer7.0阅读器支持所有CNKI文件格式，AdobeReader仅支持PDF格式 | | | | Determination of EGF in human tear by the Flow Cytometric Microsphere Assay | | | ZHANG Yan-li;MA Xuan*;HUANG Qiang;LI Bing;Wu Kai-li.Key Laboratory of Ophthalmology;Zhongshan Ophthalmic Center state Sun yat-sen University.Guangzhou 510060;China.*Department of Ophthalmology;Yunnan Second People's Hospital.Kunming 650021;China. | | | Objective To investigate the application of xMAP (flexible multi-analyte profiling) technology compared to enzyme-linked immunosorbent assay (ELISA) in the assay of EGF in human tear.Methods Non-reflex tear from twenty-four healthy humans were collected under slit-lamp biomicroscope.The EGF concentration in human tear was measured by xMAP.Meanwhile,the ELISA method was used for comparison.All data was analyzed by the statistic software SPSS.Results The EGF concentration determined by xMAP was (4.32 ± 2.57)ng/ml (range:0.96～9.76ng/ml) in humans (age:18～33 yrs).It was 8.78 ± 5.35ng/ml (range:2.45～20.39ng/ml) when measured by ELISA.The correlation coefficient (R2) of the data assayed by two methods was 0.8508 (p=0.000).Conclusion There is a good relation between xMAP and ELISA in the determination of the EGF in human tear.The xMAP technology can be used in the assay of trace proteins in human tear.
【Keyword】：epidermal growth factor;tear;enzyme-linked immunosorbent assay;flow cytometric microsphere assay |
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