| | | | | Detection of RASSF1A promoter hypermethylation in serum from gastric and colorectal adenocarcinoma patients | | | AIM:To evaluate the diagnostic role of serum RASSF1A promoter hypermethylation in gastric and colorectal ad- enocarcinoma. METHODS:Methylation-specific polymerase chain reac- tion (MSPCR) was used to examine the promoter meth- ylation status of the serum RASSF1A gene in 47 gastric adenocarcinoma patients, 45 colorectal adenocarcinoma patients, 60 patients with benign gastrointestinal dis- ease (30 with benign gastric disease and 30 with benign colorectal disease), and 30 healthy donor controls. Apaired study of RASSF1A promoter methylation status in primary tumor, adjacent normal tissue, and postopera- tive serum were conducted in 25 gastric and colorectal adenocarcinoma patients who later were underwent sur- gical therapy. RESULTS:The frequencies of detection of serum RASSF1A promoter hypermethylation in gastric (34.0%) and colorectal (28.9%) adenocarcinoma patients were significantly higher than those in patients with benign gastric (3.3%) or colorectal (6.7%) disease or in healthy donors (0%) (P < 0.01). The methylation status of RASSF1A promoter in serum samples was consistent with that in paired primary tumors, and the MSPCR results for RASSF1A promoter methylation status in paired preoperative samples were consistent with those in postoperative serum samples. The serum RASSF1A promoter hypermethylation did not correlate with patient sex, age, tumor differentiation grade, surgical therapy, or serum carcinoembryonic antigen level. Although the serum RASSF1A promoter hypermethylation frequency tended to be higher in patients with distant metastases, there was no correlation between methylation status and metastasis. CONCLUSION:Aberrant CpG island methylation within the promoter region of RASSF1A is a promising biomark- er for gastric and colorectal cancer.
【分类号】：R735
【DOI】：CNKI:SUN:ZXXY.0.2008-19-021
【正文快照】：
INTRODUCTION Gastric and colorectal cancers are two of the most com- mon causes of cancer-related death worldwide. Develop- ment of efficient diagnostic methods to enable their early detection plays an essential role in increasing the survival rate of pat… | | |
| | |  推荐 CAJ下载  PDF下载 | | | CAJViewer7.0阅读器支持所有CNKI文件格式，AdobeReader仅支持PDF格式 | | | | Detection of RASSF1A promoter hypermethylation in serum from gastric and colorectal adenocarcinoma patients | | | Yu-Cai Wang;Zheng-Hong Yu;Chang Liu;Li-Zhi Xu;Wen Yu;Jia Lu;Ren-Min Zhu;Guo-Li Li;Xin-Yi Xia;Xiao-Wei Wei;Hong-Zan Ji;Heng Lu;Yong Gao;Wei-Min Gao;Long-Bang ChenDepartment of Medical Oncology;Jinling Hospital;Nanjing 210002;Jiangsu Province;China Medical School of Nanjing University;Nanjing 210093;Jiangsu Province;China Department of Experimental Radiation Oncology;The University of Texas M.D. Anderson Cancer Center;The University of Texas Graduate School of Biomedical Sciences at Houston;Houston TX 77030;United States Department of Molecular Pathology;The University of Texas M. D. Anderson Cancer Center;Houston TX 77030 United States Department of Gastroenterology;Jinling Hospital;Nanjing 210002;Jiangsu Province;China Institute of General Surgery;Jinling Hospital;Nanjing 210002;Jiangsu Province;China Institute of Laboratory Medicine;Jinling Hospital;Nanjing 210002;Jiangsu Province;China Department of Environmental Toxicology;The Institute of Environmental and Human Health;Texas Tech University;Lubbock TX 79409;United States | | | AIM:To evaluate the diagnostic role of serum RASSF1A promoter hypermethylation in gastric and colorectal ad- enocarcinoma. METHODS:Methylation-specific polymerase chain reac- tion (MSPCR) was used to examine the promoter meth- ylation status of the serum RASSF1A gene in 47 gastric adenocarcinoma patients, 45 colorectal adenocarcinoma patients, 60 patients with benign gastrointestinal dis- ease (30 with benign gastric disease and 30 with benign colorectal disease), and 30 healthy donor controls. Apaired study of RASSF1A promoter methylation status in primary tumor, adjacent normal tissue, and postopera- tive serum were conducted in 25 gastric and colorectal adenocarcinoma patients who later were underwent sur- gical therapy. RESULTS:The frequencies of detection of serum RASSF1A promoter hypermethylation in gastric (34.0%) and colorectal (28.9%) adenocarcinoma patients were significantly higher than those in patients with benign gastric (3.3%) or colorectal (6.7%) disease or in healthy donors (0%) (P < 0.01). The methylation status of RASSF1A promoter in serum samples was consistent with that in paired primary tumors, and the MSPCR results for RASSF1A promoter methylation status in paired preoperative samples were consistent with those in postoperative serum samples. The serum RASSF1A promoter hypermethylation did not correlate with patient sex, age, tumor differentiation grade, surgical therapy, or serum carcinoembryonic antigen level. Although the serum RASSF1A promoter hypermethylation frequency tended to be higher in patients with distant metastases, there was no correlation between methylation status and metastasis. CONCLUSION:Aberrant CpG island methylation within the promoter region of RASSF1A is a promising biomark- er for gastric and colorectal cancer.
【Keyword】：Gastric cancer;Colorectal cancer;Gene methylation;RASSF1A |
| | | | | | 1 | Liu L, Tommasi S, Lee DH, Dammann R, Pfeifer GP; Control of microtubule stability by the RASSF1A tumor suppressor [M];Onco- gene; 2003年 | | 2 | Vos MD, Martinez A, Elam C, Dallol A, Taylor BJ, Latif F, Clark GJ; A role for the RASSF1A tumor suppressor in the reg- ulation of tubulin polymerization and genomic stability [M];Cancer Res; 2004年 | | 3 | Fujiwara K, Fujimoto N, Tabata M, Nishii K, Matsuo K, Hotta K, Kozuki T, Aoe M, Kiura K, Ueoka H, Tanimoto M; Identification of epigenetic aberrant promoter methylation in serum DNA is useful for early detection of lung cancer [M];Clin Cancer Res; 2005年 |
|
|
|