| | | | | Inhibitory effect of dimeric β peptide on the recurrence and metastasis of hepatocellular carcinoma in vitro and in mice | | | AIM:To block the adhesion of tumor cells to the extra- cellular matrix, and prevent tumor metastasis and recur- rence, the dimer of the β peptide (DLYYLMDLSYSMKG- GDLYYLMDLSYSMK, β2) was designed and synthesized and its anti-adhesion and anti-invasion effects on hepa- tocellular carcinoma cells were assessed. Additionally, its influence on the metastasis and recurrence of mouse hepatocellular carcinoma was measured. METHODS:The anti-adhesion effect of β2 on the highly metastatic hepatocellular carcinoma cell line HCCLM6 cells and fibronectin (FN) was assayed by the MTT as- say. The inhibition of invasion of HCCLM6 cells by β2 was observed using a Transwell (modified Boyden chamber) and matrigel. Using the hepatocellular carcinoma metas- tasis model and LCI-D20 nude mice, the influence of β2 on the metastasis and recurrence of hepatocellular carci- noma after early resection was investigated. RESULTS:HCCLM6 cells co-incubated with 100 mmol/L, 50 mmol/L, 20 mmol/L or 10 mmol/L β2 for 3 h showed an obvious decrease in adhesion to FN. The adhesion inhibition ratios were 11.8%, 21.7%, 29.6% and 48.7%, respectively. Additionally, HCCLM6 cells cultured with 100 mmol/L β2 had a dramatic decrease in cell invasion. β2 was also observed to inhibit the incisal edge recur- rence and the distant metastasis of nude mice hepato- cellular carcinoma after early resection (P < 0.05). CONCLUSION:The β2 peptide can specifically block the adhesion and invasion of HCCLM6 cells, and can inhibit HCC recurrence and metastasis of LCI-D20 model pos-thepatectomy in vivo. Thus, β2 should be further studied as a new anti-tumor drug.
【基金】：National High-Tech Program of China, No. 2001AA215411 and No. 2004AA215201;Shanghai Science and Technology Developing Program, No. 024319212
【分类号】：R735.7
【DOI】：CNKI:SUN:ZXXY.0.2008-19-017
【正文快照】：
INTRODUCTION Despite significant advances in the treatment of human hepatocellular carcinoma (HCC) and the prevention of postoperative metastasis, the 5-year postoperative recurrence rate of HCC is still very high[1,2]. Many efforts have been made to deve… | | |
| | |  推荐 CAJ下载  PDF下载 | | | CAJViewer7.0阅读器支持所有CNKI文件格式，AdobeReader仅支持PDF格式 | | | | Inhibitory effect of dimeric β peptide on the recurrence and metastasis of hepatocellular carcinoma in vitro and in mice | | | Song-Mei Wang;Jun Zhu;Luan-Feng Pan;Yin-Kun Liu Laboratory of Molecular Biology;Shanghai Medical College;Fudan University;Shanghai 200032;China Department of Pharmacy;Shanghai Chest Hospital;Shanghai 200031;China Liver Cancer Institute;Fudan University;Shanghai 200032;China | | | AIM:To block the adhesion of tumor cells to the extra- cellular matrix, and prevent tumor metastasis and recur- rence, the dimer of the β peptide (DLYYLMDLSYSMKG- GDLYYLMDLSYSMK, β2) was designed and synthesized and its anti-adhesion and anti-invasion effects on hepa- tocellular carcinoma cells were assessed. Additionally, its influence on the metastasis and recurrence of mouse hepatocellular carcinoma was measured. METHODS:The anti-adhesion effect of β2 on the highly metastatic hepatocellular carcinoma cell line HCCLM6 cells and fibronectin (FN) was assayed by the MTT as- say. The inhibition of invasion of HCCLM6 cells by β2 was observed using a Transwell (modified Boyden chamber) and matrigel. Using the hepatocellular carcinoma metas- tasis model and LCI-D20 nude mice, the influence of β2 on the metastasis and recurrence of hepatocellular carci- noma after early resection was investigated. RESULTS:HCCLM6 cells co-incubated with 100 mmol/L, 50 mmol/L, 20 mmol/L or 10 mmol/L β2 for 3 h showed an obvious decrease in adhesion to FN. The adhesion inhibition ratios were 11.8%, 21.7%, 29.6% and 48.7%, respectively. Additionally, HCCLM6 cells cultured with 100 mmol/L β2 had a dramatic decrease in cell invasion. β2 was also observed to inhibit the incisal edge recur- rence and the distant metastasis of nude mice hepato- cellular carcinoma after early resection (P < 0.05). CONCLUSION:The β2 peptide can specifically block the adhesion and invasion of HCCLM6 cells, and can inhibit HCC recurrence and metastasis of LCI-D20 model pos-thepatectomy in vivo. Thus, β2 should be further studied as a new anti-tumor drug.
【Keyword】：β peptide;Hepatocellular carcinoma;Anti- adhesion;Invasion;Metastasis;Recurrence |
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