| | | | | TNF-α和吡格列酮对3T3-L1脂肪细胞adiponutrin mRNA表达的影响 | | | 刘莉;王佑民;丁振兴;杨明功 | | | 目的探讨肿瘤坏死因子α(TNF-α)和吡格列酮(PIO)对3T3-L1脂肪细胞中,脂肪滋养蛋白(adiponutrin ADPN)mRNA表达的影响及时间效应。方法用100μmol/L的PIO和100ng/ml的TNF-α处理不同阶段的3T3-L1脂肪细胞,RT-PCR检测ADPN的表达水平。结果在分化过程中和分化成熟的3T3-L1细胞中,TNF-α均增加ADPN的表达,PIO则可明显抑制其mRNA的表达。结论PIO和TNF-α可影响3T3-L1脂肪细胞的ADPN的表达。
【作者单位】：安徽医科大学内分泌代谢病研究所 第一附属医院内分泌科;安徽医科大学内分泌代谢病研究所;第一附属医院内分泌科;安徽医科大学内分泌代谢病研究所;第一附属医院内分泌科;安徽医科大学内分泌代谢病研究所;第一附属医院内分泌科
【关键词】：脂肪滋养蛋白;脂肪细胞,3T3-L1;肿瘤坏死因子α;吡格列酮
【基金】：安徽省自然科学基金资助项目(03043719)
【分类号】：R589
【DOI】：CNKI:SUN:ZGTL.0.2008-05-004
【正文快照】：
随着研究的深入,人们发现脂肪组织具有内分泌功能。脂肪产生的因子包括分泌性因子如瘦素、脂联素、肿瘤坏死因子α(TNF-α)和非分泌蛋白(如Perilipin)等。已经证实脂肪产生的许多蛋白在能量平衡方面有着重要的作用。脂肪滋养蛋白(adiponutrin,ADPN)是新近发现的一种非分泌蛋白[ | | |
| | |  推荐 CAJ下载  PDF下载 | | | CAJViewer7.0阅读器支持所有CNKI文件格式，AdobeReader仅支持PDF格式 | | | | Effects of pioglitazone and TNF-α on adiponutrin mRNA expression in 3T3-L1 adipocytes | | | LIU Li;WANG You-min;DING Zhen-xing;et al.Institute of Endocrinology & Metabolism;Anhui Medical University;The First Affiliated Hospital of Anhui Medical University;Hefei 230022;China | | | Objective To investigate effects of TNF-α and pioglitazone on adiponutrin mRNA(ADPN)expression in 3T3-L1 adipocytes.Methods The differentiating and full differentiated 3T3-L1 adipocytes were treated with 100ng/ml of TNF-α or 100umol/L of pioglitazone during cell differentiation.Semi-quantitative RT-PCR was used to analyze ADPN gene expression.Results After TNF-α treatment,ADPN mRNA expression was increased in 3T3-L1 cells.In contrast,pioglitazone dramatically down-regulated ADPN expression.Conclusions ADPN expression is modulated by TNF-α and pioglitazone in 3T3-L1 cells.
【Keyword】：Adiponutrin;Adipocytes,3T3-L1;TNF-α;Pioglitazone |
|
|