| | | | | 多不饱和脂肪酸对小鼠瘦素表达的影响 | | | 江津;樊超男;朱海燕;齐可民 | | | 目的探讨多不饱和脂肪酸(PUFAs)对瘦素表达的影响。方法使用4w龄C57BL/6J雌性小鼠,分别给予不同种类高脂饲料(18%脂肪,供能比为36%)喂养-高脂豆油饲料、高脂鱼油饲料和高脂豆油:鱼油(5:1)混合饲料,以正常饲料(6%脂肪来自豆油,供能比为12%)为对照,小鼠自由进食,喂养时间为4个月。同时进行了PUFAs对体外培养3T3-L1细胞分化的干预实验,在细胞被诱导分化D5,在培养基中分别加入油酸(OA,C18:1n-9)、花生四烯酸(AA,C20:4n-6)、二十碳五烯酸(EPA,C20:5n-3)和二十二碳六烯酸(DHA,22:6n-3,0.125mmol/L)培养12h。采用酶联免疫吸附试验(ELISA)观察小鼠血浆和细胞培养基中瘦素及瘦素受体(sOB-R)表达的变化。结果鱼油组、豆油:鱼油组以及豆油组之间血浆瘦素水平(15.97±1.41,10.37±0.98和5.81±2.95μg/L)均存在显著性差异(鱼油>豆油:鱼油>豆油);与正常对照组(14.96±6.62μg/L)比,鱼油组血浆瘦素水平未发生明显变化。血浆sOB-R水平在4组饲料之间未见显著性差异。细胞培养显示,与对照组(18.70±0.67μg/gprot)比,在培养基中加入EPA和DHA后瘦素表达量(30.19±7.11和28.18±4.87μg/gprot)明显增多;而加入OA和AA对瘦素表达(19.75±6.11和24.48±9.84μg/gprot)无影响。值得注意的是,未能检测到细胞培养基中sOB-R的表达。结论饲料PUFAs对小鼠瘦素表达具有调节功能,但鱼油n-3PUFAs和豆油n-6PUFAs的作用不同。
【作者单位】：首都医科大学附属北京儿童医院儿科研究所临床营养中心 北京100045
【关键词】：n-3多不饱和脂肪酸;瘦素;小鼠;3T3-L1细胞
【基金】：北京市优秀人才培养专项经费资助项目(No.20051D0300824)
【分类号】：R151
【DOI】：CNKI:SUN:YYXX.0.2008-02-017
【正文快照】：
肥胖的发生机制尚不完全清楚。1994年肥胖基因(ob)克隆成功及其表达产物瘦素功能的发现,使人们一度认为找到了肥胖发生的机制,但由于肥胖状态下瘦素抵抗的问题未解决,故对肥胖的深入研究受到了阻碍[1]。有研究表明,多不饱和脂肪酸(PUFAs),对脂肪合成、分解与聚集、脂肪细胞的分 | | |
| | |  推荐 CAJ下载  PDF下载 | | | CAJViewer7.0阅读器支持所有CNKI文件格式，AdobeReader仅支持PDF格式 | | | | EFFECTS OF POLYUNSATURATED FATTY ACIDS ON LEPTIN EXPRESSION IN MICE | | | JIANG Jin;FAN Chao-nan;ZHU Hai-yan;QI Ke-min(Clinical Nutrition Center;Pediatric Research Institute;Beijing Children’s Hospital;Capital Medical University;Beijing 100045;China) | | | Objective To investigate the effects of polyunsaturated fatty acids(PUFAs) on expression of leptin and soluble leptin receptor(sOB-R).Method Four-week-old C57BL/6J female mice,that had free access to food,were fed three different high-fat(18% by weight supplying 36% of total energy) diets:soy oil,fish oil and soy:fish oil(5:1) respectively with normal chow diet(6% of fat as soy oil supplying 12% of total energy) as control for 4 months.Meanwhile,3T3-L1 cells were used and incubated respectively with arachidonic acid(AA,C20:4n-6),eicosapentaenoic acid(EPA,C20:5n-3),docosahexaenoic acid(DHA,22:6n-3) and oleic acid(OA,18:1n-9,0.125 mmol/L) for 12 h at 5 d after differentiation.Then,blood and cell culture media were collected and leptin and sOB-R in them were measured by enzyme-linked immunosorbent assay.Results Differences were found in plasma leptin levels among three groups of mice fed high-fat diets(fish oil:15.97±1.41>soy:fish oil:10.37±0.98>soy oil:5.81±2.95 μg/L ).Compared to normal chow diet,fish oil diet did not change mouse plasma leptin concentration.Plasma sOB-R levels in four groups were similar.Culture of 3T3-L1 cells showed that addition of EPA and DHA to the media increased leptin concentrations( 30.19 ±7.11 and 28.18±4.87 μg/gprot) as compared with control(18.70±0.67 μg/g prot),whereas OA and AA did not change leptin expression(19.75±6.11 and 24.48±9.84 μg/g prot).No sOB-R expression was detected in all four groups.Conclusion PUFAs in diet can regulate leptin expression in mice,and the regulatory effects are different between fish oil n-3 PUFAs and soy oil n-6 PUFAs.
【Keyword】：n-3 polyunsaturated fatty acids;leptin;mouse;3T3-L1 |
| | | | | | 1 | Shimizu H,Oh-I S,Okada S,et al; Leptin resistance and obesity[J] [M];Endocr J; 2007年 | | 2 | Considine RV; Human leptin:an adipocyte hormone with weight-regulatory and endocrine functions[J] [M];Semin Vasc Med; 2005年 | | 3 | Winnicki M,Somers VK,Accurso V,et al; Fish-rich diet,leptin,and body mass[J] [M];Circula-tion; 2002年 | | 4 | Peyron-Caso E,Taverna M,Guerre-Millo M,et al; Dietary(n-3)polyunsaturated fatty acids up-regulate plasma leptin in insulin-resistant rats[J] [M];J Nutr; 2002年 | | 5 | Perez-Matute P,Marti A,Martinez JA,et al; Eicosapentaenoic fatty acid increases leptin secretion from primary cultured rat adipocytes:role of glucose metabolism[J] [M];Am J Physiol Regul Integr Comp Physiol; 2005年 | | 6 | Lombardo YB,Chicco AG; Effects of dietary poly-unsaturated n-3fatty acids on dyslipidemia and insulin resistance in rodents and humans[J] [M];J Nutr Biochem; 2006年 | | 7 | Takahashi Y,Ide T; Dietary n-3fatty acids affect mRNA level of brown adipose tissue un-coupling protein1,and white adipose tissue leptin and glucose transporter4in the rat[J] [M];Br J Nutr; 2000年 | | 8 | Oh-I S,Shimizu H,Sato T,et al; Molecular mechanisms associated with leptin resistance:n-3polyunsaturated fatty acids induce altera-tions in tight junction of the brain[J] [M];Cell Metab; 2005年 | | 9 | Simopoulos AP; Evolutionary aspects of diet,the omega-6/omega-3ratio and genetic variation:nutritional implications for chronic diseases[J] [M];Biomed Pharmacother; 2006年 | | 10 | Wyrwoll CS,Mark PJ,Mori TA,et al; Prevention of programmed hyperleptinemia and hyperten-sion by postnatal dietary omega-3fatty acids[J] [M];Endocrinology; 2006年 |
|
| | | | | | 1 | 沙怡梅; 鱼油可增加n-3多不饱和脂肪酸的摄入 [J];国外医学.卫生学分册; 2000年04期; 59-60 | | 2 | 杨守美; n-3多不饱和脂肪酸 [J];中国医药导报; 2006年15期; 206-207 | | 3 | 韩树萍,郭锡熔,顾筱琪; 瘦素在宫内发育迟缓婴儿追赶生长中的作用 [J];中国新生儿科杂志; 2006年03期; 22-24 | | 4 | 张洪涛,单雷,毕玉平; n-6和n-3多不饱和脂肪酸在人和动物体内的功能关系 [J];山东农业科学; 2006年02期; 121-126 | | 5 | 潘妹霞,苏宜香; 膳食脂肪酸构成对小鼠淋巴细胞功能和脂质过氧化的影响 [J];营养学报; 2004年06期; 10-14 | | 6 | 潘妹霞,苏宜香,冯翔,谭炳炎; 膳食n-6/n-3脂肪酸比值对小鼠淋巴细胞脂肪酸构成和功能的影响 [J];卫生研究; 2005年01期; 102-105 | | 7 | 宋芸,丁华,吴葆杰; n-3多不饱和脂肪酸的研究进展 [J];中国海洋药物; 2005年06期; 61-64 | | 8 | 李殿鑫,陈银基,周光宏,徐幸莲; n-3多不饱和脂肪酸分类、来源与疾病防治功能 [J];中国食物与营养; 2006年06期; 53-55 | | 9 | 李桂玲,金庆华,何世文; 中国淡水鱼油的研究——Ⅳ降低鱼油过氧化值方法的研究 [J];中国海洋药物; 1998年01期; 42-46 | | 10 | 蔡妙颜,李冰,袁向华; 膳食中的脂肪酸平衡 [J];粮油食品科技; 2003年02期; 37-39 |
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