| | | | | rhBMP-2/无定形磷酸钙纳米缓释微粒成骨活性的实验研究 | | | 沈素祥;吴小涛;张绍东;王皓宇;李延报;杨惠林;唐天驷 | | | 目的探讨无定形磷酸钙(ACP)对rhBMP-2的缓释作用,检测rhBMP-2/ACP纳米缓释微粒的成骨活性。方法用扫描电镜观察已制备rhBMP-2/ACP缓释微粒的大小、形态;测定rhBMP-2的体外释放情况并描绘曲线;用MTT法检测缓释微粒对兔骨髓间充质干细胞(MSC)增殖情况的影响;用碱性磷酸酶(ALP)试剂盒检测细胞ALP活性以反映缓释微粒对其分化的影响,并与ACP的作用进行比较;将缓释微粒植入大鼠股部肌袋,通过X线、组织形态学观察评价缓释微粒的异位成骨能力。结果缓释微粒大小为100nm左右,具有典型的无定形球形面貌。开始时为快速释放期,随后呈缓慢持续释放。缓释微粒能显著促进MSC的增殖和分化,植入大鼠股部肌袋12周,材料大部分降解,有明显的骨形成。结论ACP可作为rhBMP-2合适的缓释载体材料,生成的纳米缓释微粒具有良好的降解性能和成骨活性。
【作者单位】：东南大学附属中大医院骨科 江苏南京210009(沈素祥;吴小涛;张绍东;王皓宇);南京工业大学材料与工程学院 江苏南京210009(李延报);苏州大学附属第一医院骨科 江苏苏州215006(杨惠林;唐天驷)
【关键词】：rhBMP-2;无定形磷酸钙;纳米微球;骨髓间充质干细胞;异位成骨能力
【基金】：江苏省卫生厅“135工程”重点学科开放课题(K0618)
【分类号】：R318.08
【DOI】：CNKI:SUN:SGLC.0.2008-03-007
【正文快照】：
骨形态发生蛋白(bone morphogenetic protein,BMP)是广泛存在于骨基质中的一种酸性蛋白,能诱导间充质干细胞向成骨细胞分化,是目前已知作用最强的促成骨因子,其效果已得到大量的实验和临床验证[1～3],但BMP在体内很容易被体液稀释及各种蛋白酶所降解,需合适的载体使其缓慢释放 | | | | | |  推荐 下载CAJ全文  下载PDF全文 | | | CAJViewer7.0阅读器支持所有CNKI文件格式，AdobeReader仅支持PDF格式 | | | | Experimental study on the osteogenic activity of rhBMP-2 loaded amorphous calcium phosphate slow release microsphere | | | SHEN Su-xiang1;WU Xiao-tao1;ZHANG Shao-dong1;WANG Hao-yu1;LI Yan-bao2;YANG Hui-lin3;TANG Tian-si3 (1.Department of Orthopedic;Zhongda Hospital of Southeast University;Nanjing 210009;Jiangsu;China;2.College of Materials Science and Engineering;Nanjing University of Technology;Nanjing 210009;Jiangsu;China;3. Department of Orthopaedic;The First Affiliated Hospital of Soochow University;Suzhou 215006;Jiangsu;China) | | | Objective To study the effect of amorphous calcium phosphate(ACP) on the slow release activity of rhBMP-2 microspheres and evaluate its osteogenic activity. Methods The size and appearance of the microspheres were observed by scanning electron microscope. The release profile of rhBMP-2/ACP was described with curve figure. The effect of the slow release microsphere on the proliferation of rabbit bone marrow stem cells(MSC) was measured by cell culture technique and MTT colorimetric assay. The ALP kit was used to evaluate the ALP activity of the cells and show the effect of the microsphere on differentiation of the cells compared with that of ACP only. The rhBMP-2/ACP or ACP microspheres were implanted bilaterally into leg muscle pouches of rats to observe ectopic bone formation activity with histological and X-ray measure. Results The diameter of rhBMP-2/ACP microspheres were about 100 nm with amorphous sphere appearance. The rhBMP-2/ACP microspheres released rapidly at the beginning , then released lasting slowly. The microspheres could significantly promote proliferation and differentiation of MSC. Conclusion It is demonstrated that ACP is an appropriate carrier material for rhBMP-2 to achieve slow release,the produced microspheres provides good slow release and osteogenic activity.
【Keyword】：rhBMP-2;amorphous calcium phosphate;microsphere;bone mesenchymal stem cells;ectopic bone formation |
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