收藏本站
收藏 | 投稿 | 手机打开
二维码
手机客户端打开本文

Purification and Characterization of a Novel Hydrolase That Can Specifically Degrade the Polysaccharide Isolated from Green Seaweed Ulva prolifera

LI Jiaxin  ZHANG Pei  HONG Liang  LIU Chenguang  
【摘要】:The extracellular polysaccharide hydrolase-producing strain EP-1 was isolated from seawater and identified as Paenibacillus pabuli. Furthermore, a homogeneous extracellular polysaccharide hydrolase from Paenibacillus pabuli EP-1 was purified by combining ion-exchange chromatography and size exclusion chromatography with a purification fold of 90.69 and recovery of 16.23%. Characterization of the purified polysaccharide hydrolase revealed a molecular mass of 38 k Da and optimum activity at 45℃ and pH 6.0. The polysaccharide hydrolase maintained its stability within a wide range of pH(3.0–12.0) and thermal stability when the temperature was below 50℃. The presence of Hg~(2+), Fe~(2+), Mn~(2+), Co~(2+) and SDS notably decreased hydrolase activity, and organic solvents such as formaldehyde, acetone, DMF and acetonitrile completely inhibited hydrolase activity. The purified hydrolase had no activity on agar, carrageenan, gellan gum, sodium alginate, or starch, but effectively hydrolyzed the polysaccharide from Ulva prolifera. The Km and Vmax values of this hydrolase were 43.84 mg m L~(-1) and 4.33 mg m L~(-1) min~(-1), respectively. The sequence analysis with quantitative time-of-flight mass spectrometry indicated that the hydrolase was an endoglucanase.

知网文化
【相似文献】
中国期刊全文数据库 前12条
1 LI Jiaxin;ZHANG Pei;HONG Liang;LIU Chenguang;;Purification and Characterization of a Novel Hydrolase That Can Specifically Degrade the Polysaccharide Isolated from Green Seaweed Ulva prolifera[J];Journal of Ocean University of China;2019年01期
2 ;Copper ions inactivate S-ade-nosylhomocysteine hydrolase[J];Chinese Science Bulletin;2002年14期
3 ;Isolation, purification and characterization of a new organphosphorus hydrolase OPHC2[J];Chinese Science Bulletin;2004年03期
4 ;Cloning and expression of ophc2,a new organphosphorus hydrolase gene[J];Chinese Science Bulletin;2004年12期
5 辛永平;孔文涛;陆文伟;孔健;;Paenibacillus sp.K1 α-半乳糖苷酶酶学性质[J];山东大学学报(理学版);2015年01期
6 ;Cloning of Bile Salt Hydrolase Gene and Its Expression in Lactic Acid Bacteria[J];Journal of Northeast Agricultural University(English Edition);2011年02期
7 李晓晖;俞勇;李会荣;张琳;蒋欣音;任大明;;Studies on culture condition and extracellular hydrolase of psychrophilic bacteria from Arctic sea ice[J];Chinese Journal of Polar Science;2008年01期
8 ;Expression,Purification and Crystal Structure of a Truncated Acylpeptide Hydrolase from Aeropyrum pernix K1[J];Acta Biochimica et Biophysica Sinica;2005年09期
9 赵芳;杨永清;刘亭君;周和治;;产胆固醇氧化酶类芽孢杆菌的复合诱变育种[J];中国酿造;2010年11期
10 Merja Lusa;Jukka Lehto;Hanna Aromaa;Jenna Knuutinen;Malin Bomberg;;Uptake of radioiodide by Paenibacillus sp., Pseudomonas sp.,Burkholderia sp. and Rhodococcus sp. isolated from a boreal nutrient-poor bog[J];Journal of Environmental Sciences;2016年06期
11 丁雨;陆现彩;周跃飞;陆建军;王汝成;;多粘芽孢杆菌(Paenibacillus polymyxa)吸附Cu~(2+)的实验研究[J];高校地质学报;2007年04期
12 赵洪新,刘旭明,谢宝恩,陈三凤;Paenibacillus massiliensis T7固氮基因簇nifBHDKENX的克隆、序列分析及铵和氧对nifB启动子的调控[J];中国科学C辑:生命科学;2005年05期
中国重要会议论文全文数据库 前10条
1 Zi-Xing Dong;Juan Zhang;Hua-Zhong Li;Guo-Cheng Du;Jian Chen;;Cloning and expression of a bile salt hydrolase(bsh) gene from Lactobacillus plantarum[A];第八届中国酶工程学术研讨会论文集[C];2011年
2 Hao-ran Wang;Hong Zhang;Hong Yue;Ge Chen;Zhi Wang;Lei Wang;;Asymmetric aldol addition reactioncatalyzed by hydrolase in organic solvent[A];第九届中国酶工程学术研讨会论文摘要集[C];2013年
3 Na Yang;Zhenyu Yu;Yi Wang;Menglong Hu;Chaopei Liu;Mingzhu Wang;Rui-Ming Xu;;Novel protein-RNA interaction motifs involving hydrolase-related and WD40-like domains[A];中国生物化学与分子生物学会2016年全国学术会议论文集[C];2016年
4 周康;贾宁;胡晨;江永亮;杨捷频;陈宇星;李胜;李卫芳;周丛照;;Crystal structure of juvenile hormone epoxide hydrolase from Bombyx mori[A];中国生物化学与分子生物学会第十一次会员代表大会暨2014年全国学术会议论文集——专题报告一[C];2014年
5 Pengjun Shi;Tiezheng Yuan;Huoqing Huang;Xiaoyan Chen;Yingguo Bai;Huiying Luo;Peilong Yang;Bin Yao;;A Bifunctional Xylanase-Glucanase with a Single Catalytic Domain from Paenibacillus sp.E18[A];第八届中国酶工程学术研讨会论文集[C];2011年
6 Mingchao Ma;Cuicui Wang;Yanqin Ding;Li Li;Delong Shen;Xin Jiang;Dawei Guan;Fengming Cao;Huijun Chen;Ruihua Feng;Xuan Wang;Yifan Ge;Liangtong Yao;Xiaohui Bing;Xiaohong Yang;Jun Li;Binghai Du;;Complete Genome Sequence of Paenibacillus polymyxa SC2,a Strain of Plant Growth Promoting Rhizobacteria with BroadSpectrum Antimicrobial Activity[A];第十一届全国土壤微生物学术讨论会暨第六次全国土壤生物与生物化学学术研讨会第四届全国微生物肥料生产技术研讨会论文(摘要)集[C];2010年
7 ;Cloning and Characterization of a novel Mannanase from Paenibacillus sp.BME-14[A];基因开启未来:新时代的遗传学与科技进步——湖北省遗传学会第八次代表大会暨学术讨论会论文摘要汇编[C];2009年
8 胡秀芳;;Transfer of Bacillus mucilaginosus and Bacillus edaphicus to the genus Paenibacillus with emended descriptions of Paenibacillus mucilaginosus comb.nov.and Paenibacillus edaphicus comb.nov.[A];2008年中国微生物学会学术年会论文摘要集[C];2008年
9 Wang Zhi;Dun Bao-Qing;Lu Ming;;Eukaryotic Secrete fusion expression of Rhizopus oryzae glucoamylase and Paenibacillus sp.xylanase[A];第五届全国微生物资源学术暨国家微生物资源平台运行服务研讨会论文摘要集[C];2013年
10 Mengping Wei;Jian Zhang;Moye Jia;Chaojuan Yang;Yunlong Pan;Shuaiqi Li;Yiwen Luo;Junyuan Zheng;Jianguo Ji;Jianguo Chen;Xinli Hu;Jingwei Xiong;Yun Shi;Chen Zhang;;Alpha/beta-hydrolase domain-containing 6(ABHD6) negatively regulates the surface delivery and synaptic function of AMPA receptors[A];中国神经科学学会第十二届全国学术会议论文集[C];2017年
中国博士学位论文全文数据库 前1条
1 李欧;Paenibacillus elgii B69胞外多糖结构鉴定及生物合成途径研究[D];浙江大学;2014年
中国硕士学位论文全文数据库 前7条
1 夏天;Paenibacillus sp.Aloe-11全基因组测序及其比较基因组学研究[D];西南大学;2012年
2 赵婧;Paenibacillus polymyxa JSa-9的抗菌物质高产菌株遗传选育研究[D];南京农业大学;2012年
3 王剑峰;Paenibacillus mucilaginosus KNP414全基因组测序及分析[D];浙江理工大学;2011年
4 刘欣;Paenibacillus sp.E18双功能木聚糖酶基因克隆及性质研究[D];中国农业科学院;2009年
5 张福特;五株芽胞杆菌鉴定[D];海南大学;2013年
6 赵芳;利用微生物生产胆固醇氧化酶的研究[D];广西大学;2006年
7 孙长坡;拮抗菌Paenibacillus polymyxa Cp-S316菌株的分离鉴定与活性物质的提取纯化及性质研究[D];山东农业大学;2003年
中国知网广告投放
 快捷付款方式  订购知网充值卡  订购热线  帮助中心
  • 400-819-9993
  • 010-62982499
  • 010-62783978