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Identification of Odor Volatile Compounds and Deodorization of Paphia undulata Enzymatic Hydrolysate

CHEN Deke  CHEN Xin  CHEN Hua  CAI Bingna  WAN Peng  ZHU Xiaolian  SUN Han  SUN Huili  PAN Jianyu  
【摘要】:Unfavorable fishy odour is an inevitable problem in aquatic products. In the present study, headspace solid-phase microextraction gas chromatography mass spectrometry(HS-SPME-GC-MS) analysis of volatiles from untreated samples and three deodorized samples(under the optimal conditions) of Paphia undulata enzymatic hydrolysate revealed that the compounds contributing to the distinctive odor were 1-octen-3-ol, n-hexanal, n-heptanal, 2,4-heptadienal, and 2,4-decadienal, whereas n-pentanal, n-octanal, n-octanol, benzaldehyde, 2-ethylfuran and 2-pentylfuran were the main contributors to the aromatic flavor. The deodorizing effects of activated carbon(AC) adsorption, yeast extract(YE) masking and tea polyphenol(TP) treatment on a P. undulata enzymatic hydrolysate were investigated using orthogonal experiments with sensory evaluation as the index. The following optimized deodorization conditions were obtained: AC adsorption(35 mg mL~(-1), 80℃, 40 min), YE masking(7 mg mL~(-1), 45℃, 30 min) and TP treatment(0.4 mg mL~(-1), 40℃, 50 min). AC adsorption effectively removed off-flavor volatile aldehydes and ketones. YE masking modified the odor profile by increasing the relative contents of aromatic compounds and decreasing the relative contents of aldehydes and ketones. The TP treatment was not effective in reducing the odor score, but it significantly reduced the relative content of aldehydes while increasing that of alkanes. It is also notable that TP effectively suppressed trimethylamine(TMA) formation in a P. undulate hydrolysate solution for a period of 72 h.

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