【摘要】：[Objectives] To find out the more suitable substrate for seed germination and seedling growth by comparing the effects of different substrates on seed germination. [Methods] Taking the seeds collected in Nyingchi Lulang area of Tibet as the research object, the germination effects(germination percentage, germination potential, germination index) and seedling growth status(primary root number, secondary root number, main root length, root-shoot ratio, seedling height) were measured and compared. [Results] The germination effect of Piptanthus nepalensis seeds was different in different substrates, and the germination rate, germination potential and germination index of P. nepalensis seeds in vermiculite were better than those in humus soil and perlite; the growth status of seedlings in different substrates was different, the number of primary and secondary roots in vermiculite substrate was significantly larger than that in the other two substrates, and the seedling growth in vermiculite was also better than that in the other two substrates; in comparing the main root length and root-shoot ratio, it was found that the main root length in perlite was longer than that in the other two substrates, and the root-shoot ratio was also higher than that in the other two substrates. [Conclusions] The seed germination effect and seedling growth status of P. nepalensis in different substrates were different, and there were significant differences. Vermiculite substrate had better effect on seed germination and growth, and vermiculite substrate could better promote seed germination and seedling growth. Vermiculite substrate was a good choice for seedling cultivation of P. nepalensis.