【摘要】：[Objectives] To establish a method for the determination of gallic acid in Shuijia Zhixue capsule and Rotala rotundifolia. [Methods] High performance liquid chromatography(HPLC) and Phenomenex C_(18) column were used, the mobile phase was 0.1% phosphoric acid∶methanol=85∶15, the flow rate was 0.5 mL/min, and the detection wavelength was 270 nm. [Results] In the range of(2-25) μg/mL, the concentration of gallic acid had a good linear relationship with the peak area, r=0.990 8; the average recovery was 96.88%. [Conclusions] The method was simple, sensitive and reproducible. It provided a basis for the analysis of gallic acid in Rotala rotundifolia and Shuijia Zhixue capsule and the establishment of quality detection methods in the future.