| | | | | 普罗布考抑制氧化低密度脂蛋白诱导人脐静脉内皮细胞Fractalkine的表达 | | | 欧柏青;姜德谦;张社兵;张小炼;刘虹;田轶伦 | | | 目的:探讨氧化低密度脂蛋白(ox-LDL)诱导人脐静脉内皮细胞(HUVEC)产生趋化因子Fracta-lkine及普罗布考的抑制作用。方法:用不同浓度的ox-LDL刺激HUVEC及用不同浓度普罗布考和PDTC预处理细胞后再用ox-LDL刺激,半定量RT-PCR方法检测Fractalkine和NF-κB p65的mRNA的表达。结果:未用ox-LDL刺激的HUVEC不表达Fractalkine,分别用25、50、100 mg/L浓度的ox-LDL刺激后,Fractalkine mRNA的表达呈浓度依赖性增加,与空白对照组比较分别增加384.58%(P<0.01)、484.09%(P<0.01)、558.26%(P<0.01),同时NF-κB p65 mRNA的表达分别增加15.71%(P>0.05),41.73%(P<0.01),66.72%(P<0.01)。40μmol/L PDTC预处理后,再用50 mg/L ox-LDL刺激HUVEC,NF-κB p65 mRNA的表达下降25.61%(P<0.01),分别用20、40、80μmol/L的普罗布考干预后,NFκ-B p65 mRNA的表达分别下调15.52%(P<0.05)、24.92%(P<0.01)、34.93%(P<0.01);同时,Fractalkine mRNA的表达则分别下调32.22%(P<0.01)、10.07%(P>0.05)、20.59%(P<0.01)、33.60%(P<0.01)。结论:ox-LDL可以通过NFκ-B p65诱导HUVEC表达Fractalkine,而普罗布考则可以抑制Fractalkine的表达,这种作用可能与抑制NF-κB p65有关。
【作者单位】：中南大学湘雅二医院心内科;中南大学湘雅二医院心内科;中南大学湘雅二医院心内科;中南大学湘雅二医院心内科;中南大学湘雅二医院心内科;中南大学湘雅二医院心内科 长沙;410011现在湖南省人民医院心内科(长沙;410005);长沙;410011;长沙;410011;长沙;410011;长沙;410011;长沙;410011
【关键词】：Fractalkine;核转录因子κBp65;普罗布考;氧化低密度脂蛋白;人脐静脉内皮细胞
【分类号】：R96
【DOI】：CNKI:SUN:LCXB.0.2008-03-017
【正文快照】：
Fractalkine是一种近年来发现的趋化因子,可以由被激活的血管内皮细胞产生。近年来的一些研究表明它参与了动脉粥样硬化的病理生理过程。氧化低密度脂蛋白(ox-LDL)是动脉粥样硬化的危险因子之一,它能通过多种机制引起血管内皮细胞的功能障碍和损伤。但ox-LDL能否诱导血管内皮细 | | |
| | |  推荐 下载CAJ全文  下载PDF全文 | | | CAJViewer7.0阅读器支持所有CNKI文件格式，AdobeReader仅支持PDF格式 | | | | Probucol inhibits expression of fractalkine induced by oxidized low-density lipoprotein in human umbilical vascular endothelial cells | | | OU Baiqing JIANG Deqian ZHANG ShebingZHANG Xiaolian LIU Hong1 TIAN Yilun(Department of Cardiology;the Second Xiangya Hospital of Central South University;Changsha;410011;China) | | | Objective:To investigate the expression of fractalkine induced by ox-LDL and the inhibitory effect of probucol in human umbilical vascular endothelial cells(HUVECs).Method:HUVECs were incubated with ox-LDL and some of them were pretreated with different concentration of probucol,then the expression of fractalkine mRNA and NF-κB p65 mRNA in HUVECs were determined by semi-quantitative RT-PCR.Result:There was no expression of fractalkine in untreated HUVECs. The expressions of fractalkine mRNA and NF-κB p65 mRNA in HUVECs were upregulated by ox-LDL(25,50,100mg/L) in a concentration-dependent manner.Pretreated by PDTC(40μmol/L) and probucol(20,40,80μmol/L),the expressions of fractalkine mRNA were significantly inhibited with down-regulation of NF-κB p65 mRNA.Conclusion:ox-LDL can induced the expression of fractalkine via NF-κB p65 pathway and can be inhibited by probucol.
【Keyword】：Fractalkine;Nuclear factor-κB p65;Probucol;Lectin-like oxidized low-density lipoprotein;Human umbilical vascular endothelial cells |
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