| | | | | 七氟醚对兔肺缺血-再灌注损伤中血红素氧合酶-1的影响 | | | 胡明品;李兴旺;陈玲阳;连庆泉 | | | 目的探讨七氟醚对兔肺缺血-再灌注损伤的血红素氧合酶-1(heme oxygenase-1,HO-1)的影响。方法健康雄性日本大耳白兔24只,随机分成四组(n=6)。假手术组(S组):开胸游离左肺门后,未行缺血-再灌注处理;缺血-再灌注组(IR组):阻断左肺门45min后松开血管夹再灌注120min;七氟醚-缺血再灌注组(Sev-IR组):先吸入1MAC七氟醚30min后行缺血-再灌注;七氟醚组(Sev-S组):持续吸入1MAC七氟醚30min,未予缺血-再灌注处理。缺血45min,再灌注120min时处死兔,观察各组肺组织HO-1活性,肺组织湿干重比(W/D)、肺泡损伤数(IRA)及电子显微镜观察肺组织超微结构的改变。数据采用单因素方差分析。结果再灌注120min后IR组和Sev-IR组肺组织W/D、IRA值均高于S组(P<0.01),而Sev-IR组的这两个指标较IR组均有明显降低(P<0.01);再灌注120min后IR组和Sev-IR组肺组织HO-1活性分别为(489.86±72.18)和(758.67±111.15)较S组(135.58±36.47)均明显升高(P<0.01);与IR组相比,Sev-IR组肺组织HO-1活性明显升高(P<0.01)。肺组织的电镜结果显示七氟醚使IR诱导的超微结构损伤减轻。上述指标于S和Sev-S组间差异无统计学意义。结论七氟醚可增强肺组织HO-1活性,这是其对肺IR损伤发挥的保护作用的机制之一。
【作者单位】：温州医学院附属第二医院麻醉科;温州医学院附属第二医院麻醉科;温州医学院附属第二医院麻醉科;温州医学院附属第二医院麻醉科
【关键词】：七氟醚;肺;再灌注损伤;血红素氧合酶-1
【分类号】：R614
【DOI】：CNKI:SUN:JJYZ.0.2007-08-014
【正文快照】：
临床上肺缺血-再灌注(IR)损伤常发生在肺移植、心跳骤停复苏后、失血性休克、体外循环、肺动脉内膜血栓切除术和萎陷肺再膨胀等情况下,是影响患者预后的重要因素[1-3]。我们初步研究表明卤族类麻醉药七氟醚可以减轻肺IR,但其机制尚不明了。血红素氧合酶-1(heme oxygenase-1,HO-1)是一种诱导型的酶蛋白,为热休克蛋白家族成员,具有组织器官的抗氧化应激保护作用。在肺IR过程中,HO-1活性升高而对肺组织有保护作用。目前,在肺IR过程中七氟醚是否对HO-1影响尚不明确。为了进一步阐明七氟醚对肺IR的保护作用机制,本实验观察七氟醚对在体肺I… | | |  推荐 CAJ下载  PDF下载 | | | CAJViewer7.0阅读器支持所有CNKI文件格式，AdobeReader仅支持PDF格式 | | | | Effects of sevoflurane on heme oxygenase-1 in lung ischemia-reperfusion injury in rabbits | | | HU Ming-pin;LI Xing-wang;CHEN Ling-yang;LIAN Qing-quan. Department of Anesthesiology;Second Hospital of Wenzhou Medical College;Wenzhou 325027;China | | | Objective To investigate the effects of sevoflurane on heme oxygenase-1 in lung ischemia-reperfusion injury(IR)in rabbits. Method Twenty four Japanese long-ear white rabbits weighting 2.5～3.0 kg were randomly divided into four groups(n=6 each): sham operation group(S), IR group in which hilum of left lung was clamped for 45 minutes and then unclamped, Sev-IR group in which 1 minimal alveolar concentration (MAC ) sevoflurane was inhaled for 30 minutes before ischemia, and Sev-S group in which sevoflurane was inhaled for 30 minutes without IR. All the animals were killed after 120-minute reperfusion. The wet-to-dry weight ratio of lung tissue(W/D), injured alveolus rate (IRA), and the activity of HO-1 were measured. Ultrastructure of lung tissue was observed by electron microscopy. Date were analyzed using one-way ANOVA. Results After 120-minuute reperfusion, the lung W/D and IRA in IR and Sev-IR groups increased progressively and was evidently higher than those in S group (P<0.01); but compared with IR group, administration of 1 MAC sevoflurane before ischemia significantly attenuated the increase of these two indexes (P<0.01). The activity of HO-1 in IR group (489.86 ± 72.18) and Sev-IR group (758.67±111.15) was much higher than that in S group (135.58 ± 36.47) (P<0.01). The activity of HO-1 in Sev-IR group was obviously increased compared with IR group (P<0.01). IR induced severe pulmonary ultrastructural changes, which was improved by sevoflurane. All the above-mentioned parameters in the S group were not significantly different from those in the Sev-S group. Conclusions Inhalation of sevoflurane before ischemia increases the lung HO-1 activity, which was one of the mechanisms underlying the protective effects of sevoflurane on lung IR injury in rabbits.
【Keyword】：Sevoflurane;Lung;Reperfusion injury;Heme oxygenase-1 |
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