| | | | | 尾加压素受体拮抗剂对急性肺损伤的干预效应 | | | 梁子敬;赖荣德 | | | 目的探讨尾加压素受体拮抗剂(urotensin receptor antagonist,URA)对损伤肺的影响。方法健康SPF级Sprague Dawley(SD)大鼠49只,随机分为A、B、C三组:抽取7只注射生理盐水作为正常对照(A组);B、C两组各21只,二组所有大鼠用油酸复制急性肺损伤(acute lung injury,ALI)模型,B组为模型对照组,C组在模型基础上加用URA为干预组。三组均于注射后3h各抽动脉血作血气分析测定血氧分压,A组抽动脉血后全部活杀取肺,B、C两组分别于3(抽动脉血后)及12、24h三个时间段各取7只活杀取肺,右肺称湿重后烘干,左肺立即以中性福尔马林固定作病理切片。结果A组大鼠注射生理盐水后无异常表现,B、C两组注射油酸后均出现呼吸急促、活动减少或不合群、紫绀等表现,与A组相比,B、C两组动脉血氧分压(PaO2)明显下降(P<0.05),肺湿/干(W/D)比值明显升高(P<0.05),但A、B两组间差异无统计学意义(P>0.05)。C组活动能力改善较B组更快,肺组织病理符合急性肺损伤变化,随时间延长,炎症细胞、红细胞、肺水肿进行性加重,但C组红细胞渗出较B组有所减少。结论尾加压素受体拮抗剂可能对急性肺损伤动物肺损害有保护作用。
【作者单位】：广州医学院附属第一医院急诊科;广州医学院附属第一医院急诊科
【关键词】：急性肺损伤;尾加压素受体拮抗剂;肺湿/干重比;肺组织病理学变化
【基金】：广东省广州市教育局基金资助项目(1053)
【分类号】：R563.8
【DOI】：CNKI:SUN:JJYZ.0.2007-08-010
【正文快照】：
ALI是临床上常见的急症,也是急性呼吸窘迫综合征(ARDS)的早期阶段,病情进展快,死亡率高,是目前研究热点之一。虽其确切发病机制不甚明确,多种细胞、细胞因子、炎症介质形成的效应网络相互作用导致ALI/ARDS发病,是目前广为认可的机制[1]。研究发现,血管活性物质在ALI发病过程中也起着一定的作用,血管活性物质尾加压素Ⅱ(urotensinⅡ,UⅡ)在ALI血浆及支气管肺泡灌洗液中明显升高[2-3]。本研究使用尾加压素受体拮抗剂对油酸型ALI模型进行干预,探讨其在ALI中的作用。1材料与方法1.1实验动物及器材1.1.1实验动物SPF级健康SD大鼠49只… | | |  推荐 CAJ下载  PDF下载 | | | CAJViewer7.0阅读器支持所有CNKI文件格式，AdobeReader仅支持PDF格式 | | | | Effect of urotensin receptor antagonist om acute lung injury | | | LIANG Zi-jing;LAI Rong-de.Emergency Medicine Department;The First Affiliated Hospital of Guangzhou Medical College;Guangzhou 510120;China | | | Objective To investigate the effect of urotensin receptor antagonist(URA) to the damaged lung of acute lung injury(ALI). Method Forty-nine specified-pathogens free(SPF) grade Sprague Dawley(SD)rats were randomly divided into three groups:seven for group A,as normal group;the other forty two rats were divided into group B and C,twenty one rats in each group.Rats of group A were injected with normal saline as control group.Group B and C were injected with oleic acid as model group,and group C injected with URA.Three hours after injection,artery blood were drawn for blood gas analysis in all rats.All rats of group A were killed,and seven in group B and group C were killed after artery blood were taken,then another seven at twelve hours in both group B and C were killed.The rest rats of group B and group C at twenty four hours were killed.Lung tissue was taken for pathology analyse in all rats. Result Rats in group A was normal after injection.Breathless,activity reducing,misfit, cyanosis appeared in both group B and group C.Compared with group A,oxygen pressure of artery blood(PaO2) was reduced significantly(P<0.05),wet/dry ratio(W/D) was increased(P<0.05),but there was no significant difference in group B and group C(P>0.05).With time prolonged,inflammation cell and red blood cell(RBC) exudated out of alveolar,lung edema aggravated progressively,exudation of RBC was less in alveolar and lung interstitial group C than group B. Conclusions Urotensin receptor antagonist maybe have some protective function on the damaged lung of ALI.
【Keyword】：Acute lung injury;Urotensin receptor antagonist;Wet/dry ratio;Morphologic changes of lung |
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