收藏本站
《Photonics Research》 2019年09期
收藏 | 投稿 | 手机打开
二维码
手机客户端打开本文

Simultaneous dual-contrast three-dimensional imaging in live cells via optical diffraction tomography and fluorescence

CHEN LIU  MICHAEL MALEK  IVAN POON  LANZHOU JIANG  ARIF M.SIDDIQUEE  COLIN J.R. SHEPPARD  ANN ROBERTS  HARRY QUINEY  DOUGUO ZHANG  XIAOCONG YUAN  JIAO LIN  CHRISTIAN DEPEURSINGE  PIERRE MARQUET  SHAN SHAN KOU  
【摘要】:We report a dual-contrast method of simultaneously measuring and visualizing the volumetric structural information in live biological samples in three-dimensional(3D) space. By introducing a direct way of deriving the 3D scattering potential of the object from the synthesized angular spectra, we obtain the quantitative subcellular morphology in refractive indices(RIs) side-by-side with its fluorescence signals. The additional contrast in RI complements the fluorescent signal, providing additional information of the targeted zones. The simultaneous dual-contrast 3D mechanism unveiled interesting information inaccessible with previous methods, as we demonstrated in the human immune cell(T cell) experiment. Further validation has been demonstrated using a Monte Carlo model.

知网文化
【相似文献】
中国期刊全文数据库 前19条
1 CHEN LIU;MICHAEL MALEK;IVAN POON;LANZHOU JIANG;ARIF M.SIDDIQUEE;COLIN J.R. SHEPPARD;ANN ROBERTS;HARRY QUINEY;DOUGUO ZHANG;XIAOCONG YUAN;JIAO LIN;CHRISTIAN DEPEURSINGE;PIERRE MARQUET;SHAN SHAN KOU;;Simultaneous dual-contrast three-dimensional imaging in live cells via optical diffraction tomography and fluorescence[J];Photonics Research;2019年09期
2 GAO Qi;WANG HongPing;SHEN GongXin;;Review on development of volumetric particle image velocimetry[J];Chinese Science Bulletin;2013年36期
3 范靖;包括;段德芳;汪连城;刘冰冰;崔田;;High volumetric hydrogen density phases of magnesium borohydride at high-pressure:A first-principles study[J];Chinese Physics B;2012年08期
4 多丽萍;李国富;于海军;汪健;金玉奇;桑凤亭;;Efficient pulsed chemical oxygen-iodine laser with instantaneous generation of atomic iodine by volumetric discharge[J];Chinese Optics Letters;2012年09期
5 向少华,宋克慧;Teleportation of an unknown two-atom state using simultaneous interaction of two two-level atoms with cavity field[J];Chinese Optics Letters;2003年08期
6 魏敏;叶擎昊;阚瑞峰;陈兵;杨晨光;许振宇;陈祥;阮俊;范雪丽;王薇;胡迈;刘建国;;Spectroscopy system based on a single quantum cascade laser for simultaneous detection of CO and CO_2[J];Chinese Physics B;2016年09期
7 朱永;梅浩;朱涛;张洁;;Dual-wavelength FBG inscribed by femtosecond laser for simultaneous measurement of high temperature and strain[J];Chinese Optics Letters;2009年08期
8 ;Quenching rates for heat equations with coupled singular nonlinear boundary flux[J];Science in China(Series A:Mathematics);2008年09期
9 钟丽云,张以谟,吕晓旭,袁操今;The reconstruction of diffractive object digital hologramat a short distance[J];Chinese Optics Letters;2004年05期
10 王国鹤;赵宏宏;任秋实;李长辉;;Simulation of light delivery for photoacoustic breast imaging using the handheld probe[J];Chinese Optics Letters;2014年05期
11 刘丽娟;贾昌春;张立民;陈姣姣;陈长进;;The effect of wave function orthogonality on the simultaneous ionization and excitation of helium[J];Chinese Physics B;2013年10期
12 ;Thermal expansion of lattice parameter of (powder) silicon up to 1473 K[J];Rare Metals;2004年04期
13 ;Quantum Information Splitting Based on Current Cavity QED Techniques[J];Communications in Theoretical Physics;2008年05期
14 陈恩果;黄佳敏;郭太良;吴仍茂;;A laser beam shaper for homogeneous rectangular illumination based on freeform micro lens array[J];Optoelectronics Letters;2016年04期
15 ;Large enhancement of transient photovoltage induced by the absorption of the metal Al[J];Science China(Physics,Mechanics & Astronomy);2012年07期
16 谭铭瑞;刘庆辉;隋宁;康智慧;张里荃;张汉壮;王文全;周强;王英惠;;Studying the charge carrier properties in CuInS_2 films via femtosecond transient absorption and nanosecond transient photocurrents[J];Chinese Physics B;2019年05期
17 马瑞;张文涛;黄稳柱;李芳;;DFB fiber laser sensor for simultaneous measurement of acoustic and magnetic fields[J];Chinese Optics Letters;2018年06期
18 周睿,温午麒,蔡志强,丁欣,王鹏,姚建铨;Efficient stable simultaneous CW dual-wavelength diode-end-pumped Nd:YAG laser operating at 1.319 and 1.338 μm[J];Chinese Optics Letters;2005年10期
19 王雄;王小林;周朴;粟荣涛;耿超;李新阳;许晓军;舒柏宏;;Coherent beam combination of adaptive fiber laser array with tilt-tip and phase-locking control[J];Chinese Physics B;2013年02期
中国重要会议论文全文数据库 前7条
1 Ke LI;Jing-feng BAI;Ya-zhu CHEN;Xiang JI;;DUAL-FOCUS SCANNING IN VOLUMETRIC HIFU ABLATION:PRELIMINARY SIMULATION STUDY[A];2015全国压电和声波理论及器件技术研讨会论文集[C];2015年
2 ;The Multilevel Fast Multipole Method for Volume Integral Equations[A];2011年全国微波毫米波会议论文集(下册)[C];2011年
3 Feng Huang;Yanhong Liu;Zhaoyang Chen;Long Wang;Maofu Ye;;Polarization and Aggregation of Grains in a Dusty Plasma System[A];第十六届全国等离子体科学技术会议暨第一届全国等离子体医学研讨会会议摘要集[C];2013年
4 N.Barrett;M.Izquierdo;J.Leroy;F.Sirotti;F.Bruneval;L.Reining;N.Vast;;Band structure of reconstructed Cu_2O(-110)[A];Program and Abstract of ICAM Workshop Physics of Novel Energy Materials[C];2010年
5 XIA Ti-rui;YANG Hong-guang;LIU Wen-jun;LIU Zhen-xing;ZHAO Wei-wei;HAN Zhi-bo;;Adsorption Characteristics of H_2 on Molecular Sieves 5A at 77 K[A];Annual Report of China Institute of Atomic Energy 2013[C];2014年
6 王平;;Synthesis and Plasmon-Induced Charge-Transfer Properties of Monodisperse Gold-Doped Titania Microspheres[A];第二届中国科学院博士后学术年会暨高新技术前沿与发展学术会议程序册[C];2010年
7 Li-Jun Zhang;Jun Jiang;Hui Jiang;Jing-Jing Zhang;Xing Jin;;Improving Training-based Reflectance Reconstruction via White-balance and Link Function[A];第37届中国控制会议论文集(F)[C];2018年
中国知网广告投放
 快捷付款方式  订购知网充值卡  订购热线  帮助中心
  • 400-819-9993
  • 010-62982499
  • 010-62783978