| | | | | Polymorphism Profile of Nine Short Tandem Repeat Loci in the Han Chinese | | | <正> Nine short tandem repeat (STR) markers (D3S1358, VWA, FGA, THO1, TPOX, CSFIPO, D5S818, D13S317, and D7S820) and a sex-identification marker (Amel-ogenin locus) were amplified with multiplex PCR and were genotyped with a four-color fluorescence method in samples from 174 unrelated Han individuals in North China. The allele frequencies, genotype frequencies, heterozygosity, probability of discrimination powers, probability of paternity exclusion and Hardy-Weinberg equilibrium expectations were determined. The results demonstrated that the genotypes at all these STR loci in Han population conform to Hardy-Weinberg equilibrium expectations. The combined discrimination power (DP) was 1.05×10-10 within nine STR loci analyzed and the probability of paternity exclusion (EPP) was 0.9998. The results indicate that these nine STR loci and the Amelo-genin locus are useful markers for human identification, paternity and maternity testing and sex determination in forensic sciences.
【分类号】：Q75
【DOI】：cnki:ISSN:1672-0229.0.2003-02-008
【正文快照】：
IntroduetionMost DNA PolymorPhisms are vari助ions in the DNAsequenee that usually have no obvious effeet onthe PhenotyPe of an individual and are inheritedfrom generation to generation aeeording to Mendelianrules.They eolltribute signifieantly to the variabiLity in the DNA sequenee of individuals of a sPeeies.Among the variations,there aremany short tandemrepeats(ST珑)in the human genome.STRS即pearto be abundant throughout the human genome,oe-Curring on aver砚e every6一10 Kb(1).Altho… | | |  推荐 CAJ下载  PDF下载 | | | CAJViewer7.0阅读器支持所有CNKI文件格式，AdobeReader仅支持PDF格式 | | | | Polymorphism Profile of Nine Short Tandem Repeat Loci in the Han Chinese | | | Shuangding Li;Chunxia Yan;Yajun Deng;Ruilin Wang;Jian Wang;Huanming Yang;and Shengbin Li
Key Laboratory of National Ministry of Health for Forensic Sciences;Xi'an Jiaotong University;Xi'an 710061;China; Beijing Genomics Institute;Chinese Academy of Sciences;Beijing 101300;China; Forensic Center in Beijing Genomics Institute;Beijing 101300;China. | | | Nine short tandem repeat (STR) markers (D3S1358, VWA, FGA, THO1, TPOX, CSFIPO, D5S818, D13S317, and D7S820) and a sex-identification marker (Amel-ogenin locus) were amplified with multiplex PCR and were genotyped with a four-color fluorescence method in samples from 174 unrelated Han individuals in North China. The allele frequencies, genotype frequencies, heterozygosity, probability of discrimination powers, probability of paternity exclusion and Hardy-Weinberg equilibrium expectations were determined. The results demonstrated that the genotypes at all these STR loci in Han population conform to Hardy-Weinberg equilibrium expectations. The combined discrimination power (DP) was 1.05×10-10 within nine STR loci analyzed and the probability of paternity exclusion (EPP) was 0.9998. The results indicate that these nine STR loci and the Amelo-genin locus are useful markers for human identification, paternity and maternity testing and sex determination in forensic sciences.
【Keyword】：short tandem repeats, polymorphism, Han population |
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