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《Journal of Genetics and Genomics》 2015年12期
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A Simple CRISPR/Cas9 System for Multiplex Genome Editing in Rice

Chun Wang  Lan Shen  Yaping Fu  Changjie Yan  Kejian Wang  
【摘要】:正Generating mutants bearing multiple gene modifications is essential for determining the functions of gene family members with redundant functions,or for analyzing epistatic relationships in genetic pathways.Using conventional methods,mutants with multiple gene mutations are generated by several rounds of intercrossing plants carrying a single mutation and identification of the offspring.This process is both time-
【作者单位】State
【基金】:supported by the Agricultural Science and Technology Innovation Program
【分类号】:Q943.2

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