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Determination of Polysaccharide Content in Tibetan RHIZOMA GASTRODIAE by Phenol-Sulphoacid Method

【摘要】:[Objective] To determine the content of polysaccharide in Tibetan RHIZOMA GASTRODIAE. [Method] Phenol-sulfuric acid colorimetric method was adopted. [Results] Glucose as standard reference, the optimum conditions for the determination of polysaccharide in Tibetan RHIZOMA GASTRODIAE were as follows: 1 ml, 6% phenol, 5 ml sulfuric acid, water bath temperature of 25 ℃. The contents of C6H12O6 in RHIZOMA GASTRODIAE were in the range of 21%-33%, and the average value was 28%, which proved Tibetan RHIZOMA GASTRODIAE was of high nutritional and economic values. [Conclusion] The method adopted in this study was accurate and simple, which was suitable for evaluating the quality of Tibetan RHIZOMA GASTRODIAE.

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