收藏本站
《Translational Neuroscience and Clinics》 2016年02期
收藏 | 投稿 | 手机打开
二维码
手机客户端打开本文

Transplantation of neural progenitor cells differentiated from adipose tissue-derived stem cells for treatment of sciatic nerve injury

Shasha Dong  Na Liu  Yang Hu  Ping Zhang  Chao Pan  Youping Zhang  Yingxin Tang  Zhouping Tang  
【摘要】:Objectives: Currently, the clinical repair of sciatic nerve injury remains difficult.Previous studies have confirmed that transplantation of adipose tissue-derived stem cells promotes nerve regeneration and restoration at peripheral nerve injury sites. Methods: In this study, adipose tissue-derived stem cells were induced to differentiate into neural progenitor cells, transfected with a green fluorescent protein-containing lentivirus, and then transplanted into the lesions of rats with sciatic nerve compression injury. Results: Fluorescence microscopy revealed that the transplanted cells survived,migrated, and differentiated in rats. At two weeks post-operation, a large number of transplanted cells had migrated to the injured lesions; at six weeks post-operation, transplanted cells were visible around the injured nerve and several cells were observed to express a Schwann cell marker. Sciatic function index and electrophysiological outcomes of the transplantation group were better than those of the control group. Cell transplantation promoted the recovery of motor nerve conduction velocity and compound muscle action potential amplitude, and reduced gastrocnemius muscle atrophy.Conclusions: Our experimental findings indicate that neural progenitor cells,differentiated from adipose tissue-derived stem cells, are potential seed stem cells that can be transplanted into lesions to treat sciatic nerve injury. This provides a theoretical basis for their use in clinical applications.

手机知网App
【参考文献】
中国期刊全文数据库 前2条
1 Eun-Hee Kim;Chan Yeong Heo;;Current applications of adipose-derived stem cells and their future perspectives[J];World Journal of Stem Cells;2014年01期
2 Hiroshi Kanno;;Regenerative therapy for neuronal diseases with transplantation of somatic stem cells[J];World Journal of Stem Cells;2013年04期
【共引文献】
中国期刊全文数据库 前10条
1 Shasha Dong;Na Liu;Yang Hu;Ping Zhang;Chao Pan;Youping Zhang;Yingxin Tang;Zhouping Tang;;Transplantation of neural progenitor cells differentiated from adipose tissue-derived stem cells for treatment of sciatic nerve injury[J];Translational Neuroscience and Clinics;2016年02期
2 梁伟中;王艳梅;艾红梅;张文健;赵作钧;;低能量激光对成熟脂肪细胞去分化的实验研究[J];中华实用诊断与治疗杂志;2016年01期
3 杨光;程庆砾;李春霖;贾雅丽;王静雪;岳文;裴雪涛;;大鼠肾组织干细胞的分离培养与鉴定[J];四川大学学报(医学版);2015年05期
4 张勇;陈晓菊;王燕杰;王涛;;兔脂肪干细胞直接修复陈旧性全层兔膝关节软骨缺损[J];临床骨科杂志;2015年04期
5 张舒;罗静聪;吕青;邓雪琴;熊炳钧;;人脂肪干细胞复合猪小肠黏膜下层脱细胞基质/壳聚糖温敏凝胶体内构建组织工程脂肪的研究[J];中国修复重建外科杂志;2015年08期
6 Nowruz Najafzadeh;Banafshe Esmaeilzade;Maryam Dastan Imcheh;;Hair follicle stem cells: In vitro and in vivo neural differentiation[J];World Journal of Stem Cells;2015年05期
7 叶英;谢熙;刘筱;燕宪亮;郭秉楠;吕兰欣;胡书群;许铁;;脑脊液诱导的骨髓源性神经样细胞移植的安全性研究[J];中国急救复苏与灾害医学杂志;2015年06期
8 刘洋;尚勇;李卓;肖天舒;郝立君;;不同年龄女性脂肪干细胞生物学特性的比较[J];中国美容整形外科杂志;2015年06期
9 亢婷;王刚;刘毅;刘刚强;;血管内皮生长因子165基因修饰的人脂肪间充质干细胞与改性丝素蛋白体内构建血管化组织工程脂肪的研究[J];兰州大学学报(医学版);2015年03期
10 黄乐;华咏梅;;釉基质蛋白对大鼠脂肪源性干细胞体外成骨分化能力的实验研究[J];口腔颌面外科杂志;2015年02期
【二级参考文献】
中国期刊全文数据库 前1条
1 杨立业,郑佳坤,刘相名,惠国桢,郭礼和;Culture of skin-derived precursors and their differentiation into neurons[J];Chinese Journal of Traumatology;2004年02期
【相似文献】
中国期刊全文数据库 前10条
1 ;In vitro differentiation of human adipose-derived mesenchymal stem cells into endothelial-like cells[J];Chinese Science Bulletin;2006年15期
2 ;Editor's Choice—Differentiation of adipose stromal cells into neuron-like cells[J];Neural Regeneration Research;2011年13期
3 Jie-fei Shen;Atsunori Sugawara;Joe Yamashita;Hideo Ogura;Soh Sato;;Dedifferentiated fat cells: an alternative source of adult multipotent cells from the adipose tissues[J];International Journal of Oral Science;2011年03期
4 ;Adult adipose-derived stromal cells differentiating into neurons and neuron-like cells[J];Neural Regeneration Research;2012年22期
5 Raquel Taléns-Visconti;Ana Bonora;Ramiro Jover;Vicente Mirabet;Francisco Carbonell;José Vicente Castell;María José Gómez-Lechón;;Hepatogenic differentiation of human mesenchymal stem cells from adipose tissue in comparison with bone marrow mesenchymal stem cells[J];World Journal of Gastroenterology;2006年36期
6 鲁峰;高建华;Rei Ogawa;Hiroshi Mizuro;Hiki Hykusoku;;Adipose tissues differentiated by adipose-derived stem cells harvested from transgenic mice[J];Chinese Journal of Traumatology;2006年06期
7 ;Neocartilage formation from predifferentiated human adipose derived stem cells in vivo[J];Acta Pharmacologica Sinica;2007年05期
8 ;2~(nd) International Symposium on Adipobiology and Adipopharmacology(ISAA) 23-25 October 2009,Varna,Bulgaria Organized by the Bulgarian Society for Cell Biology[J];Neural Regeneration Research;2009年08期
9 Louis Casteilla;Valérie Planat-Benard;Patrick Laharrague;Béatrice Cousin;;Adipose-derived stromal cells: Their identity and uses in clinical trials, an update[J];World Journal of Stem Cells;2011年04期
10 ;A novel ethanol-based method to induce differentiation of adipose-derived stromal cells into astrocytes[J];Neural Regeneration Research;2011年10期
中国重要会议论文全文数据库 前10条
1 辛钟成;;Augmentation Penoplasty in small penis syndrome patients;using adipose tissue derived stem cell:experimental and a pilot clinical results[A];第七次全国中西医结合男科学术会议及全国中西医结合男科提高班论文汇编及讲义[C];2011年
2 ;HUVEC can promote osteogenic differentiation of human adipose derived stem cells[A];中华医学会第三次骨质疏松和骨矿盐疾病中青年学术会议论文汇编[C];2011年
3 ;Visfatin derived from perivascular adipose tissue promotes vascular smooth muscle cell proliferation[A];中国药理学会第十次全国学术会议专刊[C];2009年
4 Jie Qian;Jing Wu;Yubin Zhang;;NT-PGC-1a promotes mitochondrial biogenesis in brown adipose tissue[A];中国生物化学与分子生物学会第十届会员代表大会暨全国学术会议摘要集[C];2010年
5 Qing Miao;Zhaoyun Zhang;Aaron M Cypess;Hongying Ye;Qiongyue Zhang;Shuo Zhang;Chuantao Zuo;Renming Hu;Yihui Guan;Yiming Li;;The prevalence and predictors of active brown adipose tissue in Chinese adults[A];中华医学会第十二次全国内分泌学学术会议论文汇编[C];2013年
6 Wei Zhang;Yuan Liu;Li Bai;Yongfu Wang;;Effect of allogenic adipose-derived stem cell transplantation on bone mass in ovariectomized female rats[A];中华医学会第五次中青年骨质疏松和骨矿盐疾病学术会议论文集[C];2013年
7 ;Research progress in Human Adipose Tissue-derived Stromal Cells[A];中国输血协会第五届输血大会论文专集(摘要篇)[C];2010年
8 周红文;段宇;杨涛;刘翠萍;覃又文;刘超;陈家伟;;肾上腺素对3T3-L1细胞Aquaporin adipose基因表达的影响[A];中华医学会第六次全国内分泌学术会议论文汇编[C];2001年
9 卢慕峻;Maurice Garcia;Thomas Fandel;Alan Shindel;Ching-Shwun Lin;王忠;Tom F. Lue;;Repairing bladder defect by adipose tissue-derived stem cell[A];第十五届全国泌尿外科学术会议论文集[C];2008年
10 黄聿;;Adipose-Vascular Communication[A];第八届海峡两岸心血管科学研讨会论文集[C];2011年
中国博士学位论文全文数据库 前1条
1 周红文;Aquapporin adipose基因表达影响因素与临床研究[D];南京医科大学;2002年
 快捷付款方式  订购知网充值卡  订购热线  帮助中心
  • 400-819-9993
  • 010-62791813
  • 010-62985026