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《China Petroleum Processing & Petrochemical Technology》 2018年04期
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Exploring the Degradation Potential of Halomonas Bacteria from Oil-contaminated Marine Environment

Wu Yanan  Liu Yingying  Xue Jianliang  Shi Ke  Gao Yu  Xiao Xiaolong  
【摘要】:The degradation potential of a diesel-degrading bacteria(HDMP1) isolated from the oil-contaminated marine environment was explored systematically by analyzing the environmental conditions and synergistic action with other dieseldegrading bacteria. The result indicated that HDMP1 was confirmed as a strain of Halomonas by the method of strain identification. And, HDMP1 showed good diesel degradation performance with a diesel degradation rate of up to 79.59%after 7 days. By analyzing the effect of environmental conditions on HDMP1, the best carbon and nitrogen sources were found to be lactose and peptone, respectively, at a pH value of 7.5 and a salinity of 4 g/(100 mL). Additionally, the synergistic effect of HDMP1 combined with other diesel-degrading bacteria was analyzed by orthogonal experimental design. The inocula of HDMP1, HDMP2, HDMP3 and HDMB3 were optimized, with the best results equating to 0.4%, 0.1%, 0.4% and0.9% in 100 mL of MSM, respectively, while the degradation rate of diesel was identified to be 73.5% within 5 days in the presence of optimum inocula.

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